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Purified anti-CXCL12 (SDF-1β) Antibody

Pricing & Availability
Clone
M4201G03 (See other available formats)
Regulatory Status
RUO
Other Names
Pre-B-cell growth-stimulating factor (PBSF), stromal cell derived factor 1 isoform beta precursor (SDF-1β), TLSF, TPAR1, SCYB12, IRH
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
M4201G03_PURE_CXCL12_SDF-1beta_Antibody_WB_091216
Total cell lysates (50ng recombinant protein per lane) from rmSDF-1β, rhSDF-1β, rmSDF-1α and rhSDF-1α were resolved by 4-12% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with anti-CXCL12 (SDF-1β) (clone M4201G03). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • M4201G03_PURE_CXCL12_SDF-1beta_Antibody_WB_091216
    Total cell lysates (50ng recombinant protein per lane) from rmSDF-1β, rhSDF-1β, rmSDF-1α and rhSDF-1α were resolved by 4-12% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with anti-CXCL12 (SDF-1β) (clone M4201G03). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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678202 100 µg 175€
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Description

SDF-1, also known as CXCL12, has different variants, which include SDF-1α, SDF-1β, and SDF-1γ derived from alternative splicing. SDF-1 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. The SDF-1/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Data suggests that SDF-1β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse SDF-1β
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565915 (BioLegend Cat. No. 678202)

Antigen Details

Structure
72 amino acids with a predicted molecular weight approximately at 8.5 kD.
Distribution

Expressed by bone marrow stromal cells, endothelial cells, epithelial cells, pericytes, dendritic cells, and fibroblasts.

Function
SDF-1 is a constitutively produced chemokine involved in lung, brain, joint, and intestine inflammation. SDF-1 is chemotactic for T, B, and cancer sells. SDF-1/CXCR4 pair plays a role in the mobilization of CD34+ progenitors to peripheral blood. SDF-1 is important in morphogenesis, angiogenesis, and cancer metastasis.
Interaction
CXCR4 and ACKR3.
Ligand/Receptor
CXCR4.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagasawa T, et al. 1994. Proc. Natl. Acad. Sci. USA 91:2305.
2. Muller A, et al. 2001. Nature 410:50.
3. Valenzuela-Fernandez A, et al. 2002. J. Biol. Chem. 277:15677.
4. De La Luz Sierra M, et al. 2004. Blood 103:2452.
5. Kortesidis A, et al. 2005. Blood 105:3793.
6. Davis DA, et al. 2005. Blood 105:4561.
7. Zlotnik A. 2008. J. Pathol. 215:211.
8. Herberg S, et al. 2013. PLoS One 8:e58207.

Gene ID
20315 View all products for this Gene ID
UniProt
View information about CXCL12 on UniProt.org
Go To Top Version: 4    Revision Date: 06-13-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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