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Purified anti-CXCL12 (SDF-1β) Antibody

Pricing & Availability
Clone
W15149A (See other available formats)
Regulatory Status
RUO
Other Names
Pre-B-cell growth-stimulating factor (PBSF), stromal cell derived factor 1 isoform beta precursor (SDF-1β), TLSF, TPAR1, SYC12, IRH
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15149A_PURE_CXCL12_Antibody_071416
50 ng of recombinant mouse CXCL12 (SDF-1β, lane 1), human CXCL12 (SDF-1β, lane 2), and mouse CXCL12 (SDF-1α, lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CXCL12 (SDF-1β, clone W15149A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection.
  • W15149A_PURE_CXCL12_Antibody_071416
    50 ng of recombinant mouse CXCL12 (SDF-1β, lane 1), human CXCL12 (SDF-1β, lane 2), and mouse CXCL12 (SDF-1α, lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-CXCL12 (SDF-1β, clone W15149A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection.
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688802 100 µg 175€
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Description

CXCL12, also known as SDF-1, has different variants, which include SDF-1α, SDF-1β, and SDF-1γ derived from alternative splicing. SDF-1 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. The SDF-1/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Data suggests that SDF-1β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse CXCL12 (SDF-1β) recombinant protein (22-93 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629642 (BioLegend Cat. No. 688802)

Antigen Details

Structure
72 amino acids with a predicted molecular weight of approximately 8.5 kD and observed molecular weight of approximately 12 kD.
Distribution
Expressed by bone marrow stromal cells, endothelial cells, epithelial cells, pericytes, dendritic cells, and fibroblasts.
Function
SDF-1 is a constitutively produced chemokine involved in lung, brain, joint, and intestine inflammation. SDF-1 is chemotactic for T, B, and cancer sells. SDF-1/CXCR4 pair plays a role in the mobilization of CD34+ progenitors to peripheral blood. SDF-1 is important in morphogenesis, angiogenesis, and cancer metastasis.
Interaction
CXCR4 and ACKR3.
Ligand/Receptor
CXCR4.
Cell Type
Endothelial cells, Epithelial cells, Dendritic cells, Fibroblasts
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagasawa T, et al. 1994. Proc. Natl. Acad. Sci. USA 91:2305.
2. Muller A, et al. 2001. Nature 410:50.
3. Valenzuela-Fernandez A, et al. 2002. J. Biol. Chem. 277:15677.
4. De La Luz Sierra M, et al. 2004. Blood 103:2452.
5. Kortesidis A, et al. 2005. Blood 105:3793.
6. Davis DA, et al. 2005. Blood 105:4561.
7. Zlotnik A. 2008. J. Pathol. 215:211.
8. Herberg S, et al. 2013. PLoS One 8:e58207.

Gene ID
6387 View all products for this Gene ID
UniProt
View information about CXCL12 on UniProt.org
Go To Top Version: 1    Revision Date: 07-20-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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