- Clone
- Poly23A05 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
637151 | 50 µL | 256€ | ||||
637152 | 200 µL | 636€ |
Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 1068 allows for binding directly to the adaptor protein GRB2. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Synthetic peptide corresponding to human EGFR phosphorylated at tyrosine 1068
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:400 - 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody (clone Poly23A05) is intended as a replacement for another antibody against the same target (clone Poly6563). Poly23A05 has a higher affinity for the target protein than Poly6563.
- RRID
-
AB_3097617 (BioLegend Cat. No. 637151)
AB_3097617 (BioLegend Cat. No. 637152)
Antigen Details
- Structure
- EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
- Distribution
-
Plasma membrane
- Function
- EGFR signaling, cell signaling
- Interaction
- GRB2, SOS1, STAT1, STAT3, JAK2, CRK
- Ligand/Receptor
- Epidermal growth factor (EGF)
- Cell Type
- Epithelial cells
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Hunter T and Cooper JA. 1981. Cell. 24:741-52.
- Gill GN and Lazar CS. 1981. Nature. 293:305-7.
- Reynolds FH, et al. 1981. Nature. 292:259-62.
- Biscardi JS, et al. 1999. J Biol Chem. 274:8335-42.
- Zhou M, et al. 2013. Cancer Res. 23:7056-67-67.
- Gene ID
- 1956 View all products for this Gene ID
- UniProt
- View information about EGFR on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All EGFR Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-EGFR Phospho (Tyr1068) | Poly23A05 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-EGFR Phospho (Tyr1068)
Whole cell extracts (15 µg total protein) from A-431 cells e...
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