- Clone
- W17130C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EIF4A1, Eukaryotic Translation Initiation Factor 4A1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
666201 | 25 µg | 81€ | ||||
666202 | 100 µg | 203€ |
EIF4A1 is an ATP-dependent RNA helicase. It can form the eIF4F complex, which is involved in translation initiation by binding with the 5' m7 GTP cap of the mRNA. It could unwind mRNA secondary structure at the 5’ UTR region and lead to the small ribosomal subunit binding and followed scanning of the initiator codon.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Hamster
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human EIF4A1 Peptide (3-19 a.a.) conjugated to KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg/mL (1:1000 - 1:20000 dilution). For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/mL (1:250 - 1:1000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 15 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/mL (1:50 - 1:100 dilution) is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2783367 (BioLegend Cat. No. 666201)
AB_2783367 (BioLegend Cat. No. 666202)
Antigen Details
- Distribution
-
Cytoplasm
- Function
- Translation initiation
- Interaction
- eIF4 complex
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Synthesis, Signal Transduction
- Gene ID
- 1973 View all products for this Gene ID
- UniProt
- View information about eIF4A1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All eIF4A1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-eIF4A1 | W17130C | WB,ICC,IHC-P,IP |
Alexa Fluor® 594 anti-eIF4A1 | W17130C | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-eIF4A1
Western blot analysis of 15 µg cell lysates from HL-60, HaCa... Immunoprecipitation analysis using purified anti-eIF4A1 anti... HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 ... Immunoprecipitation analysis using purified anti-eIF4A1 anti... IHC staining of purified anti-eIF4A1 antibody (clone W17130C... -
Alexa Fluor® 594 anti-eIF4A1
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 ...
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