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Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1248) Antibody

Pricing & Availability
Clone
Poly22A04 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine-protein kinase erbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly22A04_PURE_ErbB2_Antibody_08172
Whole cell extracts (15 µg total protein) from untreated MCF-7 cells (-) or MCF-7 cells treated with 100ng/mL of Neuregulin 1 for 5 min (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1248) (clone Poly22A04) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (Cat. No. 406401). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Equal CD340 loading was confirmed by probing membranes with a pan-reactive CD340 antibody. Lane M: Molecular weight marker
  • Poly22A04_PURE_ErbB2_Antibody_08172
    Whole cell extracts (15 µg total protein) from untreated MCF-7 cells (-) or MCF-7 cells treated with 100ng/mL of Neuregulin 1 for 5 min (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1248) (clone Poly22A04) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (Cat. No. 406401). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Equal CD340 loading was confirmed by probing membranes with a pan-reactive CD340 antibody. Lane M: Molecular weight marker
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616451 50 µL 240€
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Description

CD340, also commonly known as HER2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of CD340 in breast cancers has been shown to confer Taxol resistance, and CD340 is a therapeutic target in a number of human cancers. CD340 activation is accompanied by autophosphorylation at multiple tyrosine residues including Tyr1248.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide of human CD340 phosphorylated at Tyr1248
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068238 (BioLegend Cat. No. 616451)

Antigen Details

Structure
CD340 is a 1,259 amino acid protein with a predicted molecular weight of 137.9 kD.
Distribution

Plasma membrane

Function
Proto-oncogene
Interaction
CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Ligand/Receptor
Unlike other members of the EGFR family, CD340 does not contain a ligand binding domain
Cell Type
Leukemia
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules, Phospho-Proteins
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about ErbB2 Phospho Tyr1248 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07-31-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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