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Purified anti-FOXP3 Antibody

Pricing & Availability
Clone
W23223B (See other available formats)
Regulatory Status
RUO
Other Names
Scurfin, JM2, IPEX, PIDX
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23223B_PURE_FOXP3_IHC-P_050925
IHC staining with anti-FOXP3 (clone W23223B) was performed on formalin-fixed paraffin embedded human lymph node tissue following antigen retrieval using Citrate buffer (Cat. No. 420902). The tissue sections were incubated with Alexa Fluor® 647 secondary antibody only (panel A) or with Purified anti-FOXP3 (clone W23223B) (panel B and C) followed by Alexa Fluor® 647 goat anti-rat (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
  • A. W23223B_PURE_FOXP3_IHC-P_050925
    IHC staining with anti-FOXP3 (clone W23223B) was performed on formalin-fixed paraffin embedded human lymph node tissue following antigen retrieval using Citrate buffer (Cat. No. 420902). The tissue sections were incubated with Alexa Fluor® 647 secondary antibody only (panel A) or with Purified anti-FOXP3 (clone W23223B) (panel B and C) followed by Alexa Fluor® 647 goat anti-rat (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
  • B. W23223B_PURE_FOXP3_WB_050925
    Whole cell extracts (15 µg total protein) were harvested from human PBMCs treated (positive control) and untreated (negative control, harvested day 1) with an activation cocktail. The activation cocktail contained of anti-CD3 (Cat. No. 317354), anti-CD28 (Cat. No. 302934), human IL-2 (Cat. No. 589104), and Human TGF-β1 (Cat. No. 781804) for 5 days to induce Treg differentiation and FOXP3 expression. Cell lysates were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-FOXP3(clone W23223B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • C. W23223B_PURE_FOXP3_ICFC_050925
    Untreated human PBMCs (negative control, day 1, open histogram) or treated with activation cocktail for 5 days to induce Treg differentiation and FOXP3 expression (positive control, filled histogram) were fixed and permeabilizated using FOXP3 Fix/Perm Buffer Set (Cat. No. 421403) and then intracellularly stained with Purified anti-FOXP3 (clone W23223B) or Purified Rat IgG2a, κ isotype control (Cat. No. 400501) (dashed line, representative histogram for untreated and treated PBMC) followed by Alexa Fluor® 647 goat anti-rat (Cat. No. 405416).
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676751 25 µg 140€
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676752 100 µg 384€
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Description

Forkhead box protein P3 (FOXP3) is a transcription factor that plays a role in the development and function of regulatory T cells (Tregs), which are essential for maintaining immune tolerance and preventing autoimmune diseases. FOXP3 is responsible for the transcriptional regulation of genes involved in Treg differentiation and function, ensuring the immune system does not attack the body's own tissues. Mutations or deficiencies in the FOXP3 gene can lead to severe autoimmune disorders, such as immunodysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), characterized by widespread inflammation and multi-organ damage. Additionally, FOXP3 has been implicated in cancer biology, where it can act as a tumor suppressor in certain contexts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Fragment of Human FOXP3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using FOXP3 Fix/Perm Buffer Set (Cat. No. 421403), True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401). 

Antigen Details

Structure
The FOXP3 protein is composed of 431 amino acids and has a molecular weight of approximately 47.2 kDa
Distribution

Nuclear

Function
• Regulatory T Cell Development: Essential for the development and function of regulatory T cells (Tregs), which maintain immune tolerance and prevent autoimmune diseases.
• Transcriptional Regulation:
• Acts as a transcription factor, binding to specific DNA regions to control the expression of genes involved in immune regulation.
• Immune Response Modulation: Helps suppress the immune response to prevent excessive inflammation and autoimmunity
Interaction
• Transcription Factors: interacts with several transcription factors, including NFAT, AML-1, and c-Rel, to regulate gene expression.
• Histone Deacetylases (HDACs): recruit class I histone deacetylases (HDAC1, HDAC2, and HDAC3) to target gene promoters, modulating chromatin structure and gene expression.
• Co-repressors and Co-activators: It interacts with co-repressors like CTBP1 and co-activators such as TIP60 to fine-tune transcriptional regulation
Cell Type
Leukemia, Lymphocytes, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Zhou Z, et al. 2008. Immunology Research. 42:19
  2. Colamatteo A, et al. 2020. Front Immunol. 10:3136
  3. Golzari-Sorkheh M, et al. 2023. Clin Exp Immunol. 213:13
  4. Xie X, et al. 2015. PLoS Genet. 11:e1005251.
Regulation
• Transcriptional Control: Regulated by various transcription factors that bind to its promoter and enhancer regions.
• Epigenetic Modifications: DNA methylation and histone modifications influence FOXP3 expression by altering chromatin structure.
• Post-Translational Modifications: Phosphorylation and other modifications can affect FOXP3 stability and function.
• TCR Signaling: T cell receptor (TCR) signaling pathways can modulate FOXP3 expression and activity
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org
Go To Top Version: 1    Revision Date: 05-09-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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