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Purified anti-GATA2 Antibody

Pricing & Availability
Clone
W17242A (See other available formats)
Regulatory Status
RUO
Other Names
GATA Binding Protein 2, IMD21, NFE1B, DCML
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17242A_Purified_GATA2_Antibody_1_100120
Whole cell extracts (15 µg total protein) from MOLT-4 (negative control), HEL, K562, and KU812 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a 0.45 micron PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-GATA2 antibody, clone W17242A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Predicted GATA2 expression data was obtained from Human Protein Atlas.
  • W17242A_Purified_GATA2_Antibody_1_100120
    Whole cell extracts (15 µg total protein) from MOLT-4 (negative control), HEL, K562, and KU812 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a 0.45 micron PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-GATA2 antibody, clone W17242A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Predicted GATA2 expression data was obtained from Human Protein Atlas.
  • W17242A_Purified_GATA2_Antibody_2_041119.png
    Whole cell extracts (15 µg total protein) from MOLT-4 (negative control), HEK293 and HeLa (reduced expression controls), BeWo, and SH-SY5Y cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a .45 micron PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GATA2 Antibody, clone W17242A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • W17242A_Purified_GATA2_Antibody_3_031320
    Molt-4 cells were treated with True-Nuclear™ Transcription Factor Buffer Set, then intracellularly stained with Purified GATA2 antibody (clone W17242A) (filled histogram) or rat IgG2b, κ Purified isotype ctrl (open histogram) followed by anti-rat IgG PE secondary antibody.
  • W17242A_Purified_GATA2_Antibody_4_031320
    HEL cells were treated with True-Nuclear™ Transcription Factor Buffer Set, then intracellularly stained with Purified GATA2 antibody (clone W17242A) (filled histogram) or Purified rat IgG2b, κ isotype ctrl (open histogram) followed by anti-rat IgG PE secondary antibody.
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614001 25 µg 81€
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614002 100 µg 203€
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Description

GATA2 is a member of the GATA transription factor family. Mutations in GATA2 lead to impaired development and differentiation of hematopoietic  lineages, including dendritic cells, monocytes, B cells, and natural killer cells. Over the course of differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exhanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant partial human GATA2 protein corresponding to amino acids 1-250
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810661 (BioLegend Cat. No. 614001)
AB_2810661 (BioLegend Cat. No. 614002)

Antigen Details

Structure
GATA2 is a 480 amino acid protein with a predicted molecular weight of 51 kD.
Distribution

Widely expressed/nucleus

Function
Transcriptional activator/Regulates developmental processes in hematopoietic cells
Cell Type
Lymphocytes
Biology Area
Cell Biology, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Orkin SH. 1992. Blood. 80: 575.
  2. Bresnick EH, et al. 2010. J. Biol. Chem. 285: 31087.
  3. Collin M, et al. 2011. Nat. Rev. Immunol. 11: 575.
  4. Doré LC, et al. 2012. Blood. 119: 3724.
Gene ID
2624 View all products for this Gene ID
UniProt
View information about GATA2 on UniProt.org
Go To Top Version: 3    Revision Date: 10-01-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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