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Purified anti-Histone H3 (C-terminus) Recombinant Antibody

Pricing & Availability
Clone
1B1-B2.RecM1 (See other available formats)
Regulatory Status
RUO
Other Names
He clustered Histone 1, Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1B1-B2RecM1_Purified_AntiHistoneH3_Antibody_1_092624
Whole cell extracts (15 μg total protein per lane) from indicated cell lines were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Histone H3 (C-terminus) Recombinant (clone 1B1-B2.recM1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 1B1-B2RecM1_Purified_AntiHistoneH3_Antibody_1_092624
    Whole cell extracts (15 μg total protein per lane) from indicated cell lines were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Histone H3 (C-terminus) Recombinant (clone 1B1-B2.recM1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • 1B1-B2RecM1_Purified_AntiHistoneH3_Antibody_2_092624
    IHC staining of Purified anti-Histone H3 (C-terminus) (clone 1B1-B2.recM1) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-Histone H3 (C-terminus) Recombinant (clone 1B1-B2.recM1) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. The tissue was subsequently co-stained with Alexa Fluor® 488 anti-Vimentin (clone O91D3) (Cat. No.677809) (green) and nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured with a 40X objective and merged. Scale bar: 50 μm
  • 1B1-B2RecM1_Purified_AntiHistoneH3_Antibody_3_092624
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained either with Purified Mouse IgG1, κ Isotype Control (panels A and C) or Purified anti-Histone H3 (C-terminus) Recombinant (clone 1B1-B2.recM1) (panels B and D), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (red) for 1 hour at room temperature. Cells were subsequently co-stained with Alexa Fluor® 488 anti-Tubulin-α (clone 10D8) (Cat. No. 627906) (green) and nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 50 μm
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646851 25 µg 136€
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646852 100 µg 336€
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
This recombinant monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0125 - 0.025 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 1.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunohistochemistry use on formalin-fixed paraffin-embedded tissue (IHC-P), either antigen retrieval with Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704) is recommended.

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:

  • Fixation buffer (Cat. No. 420801) followed by 0.5 % Triton X-100
  • Fixation buffer (Cat. No. 420801) followed by 100% ice-cold methanol
  • 100% ice-cold methanol only

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Nucleus

Function
Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References
  1. Chen HM, et al. 2017. Chinese Journal of Hematology 38:744-78.
Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3 on UniProt.org
Go To Top Version: 1    Revision Date: 09-26-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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