Purified anti-human CD107a LAMP-1 Maxpar Ready Antibody anti-CD107a

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Clone: H4A3 (See other available formats)
Regulatory Status: RUO
Workshop: P PR-63; BP 473; P P008
Other Names: Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs were incubated for 5 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin, brefeldin A and 151Eu-anti-CD107a (H4A3). Cells were then fixed, permeabilized, and stained with 165Ho-anti-IFNÎ³(B27). CD8+ T cells are displayed in the analysis. Data provided by DVS Sciences.

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328635

100 µg

113€

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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8⁺ T cell and NK cell cytotoxicity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human adult adherent peripheral blood cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting⁸, immunohistochemical staining², immunofluorescence^5,7, and immunoprecipitation⁵.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563708 (BioLegend Cat. No. 328635)

Antigen Details

Structure: LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution: Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
Function: Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor: Galaptin
Cell Type: Endothelial cells, Epithelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
Gene ID: 3916 View all products for this Gene ID
UniProt: View information about CD107a on UniProt.org

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD107a Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD107a (LAMP-1)	H4A3	FC
Purified anti-human CD107a (LAMP-1)	H4A3	FC,ICC,WB,IHC-P,IP,SB
FITC anti-human CD107a (LAMP-1)	H4A3	FC
PE anti-human CD107a (LAMP-1)	H4A3	FC
Alexa Fluor® 488 anti-human CD107a (LAMP-1)	H4A3	FC
Alexa Fluor® 647 anti-human CD107a (LAMP-1)	H4A3	FC
PerCP/Cyanine5.5 anti-human CD107a (LAMP-1)	H4A3	FC
APC anti-human CD107a (LAMP-1)	H4A3	FC
Pacific Blue™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 421™ anti-human CD107a (LAMP-1)	H4A3	FC
PE/Cyanine7 anti-human CD107a (LAMP-1)	H4A3	FC
APC/Cyanine7 anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 510™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 605™ anti-human CD107a (LAMP-1)	H4A3	FC
Purified anti-human CD107a (LAMP-1) (Maxpar® Ready)	H4A3	FC,CyTOF®
Brilliant Violet 650™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 711™ anti-human CD107a (LAMP-1)	H4A3	FC
PerCP anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 785™ anti-human CD107a (LAMP-1)	H4A3	FC
PE/Dazzle™ 594 anti-human CD107a (LAMP-1)	H4A3	FC
TotalSeq™-A0155 anti-human CD107a (LAMP-1)	H4A3	PG
TotalSeq™-C0155 anti-human CD107a (LAMP-1)	H4A3	PG
TotalSeq™-B0155 anti-human CD107a (LAMP-1)	H4A3	PG
APC/Fire™ 750 anti-human CD107a (LAMP-1) Antibody	H4A3	FC
PE/Cyanine5 anti-human CD107a (LAMP-1)	H4A3	FC
Spark Blue™ 574 anti-human CD107a (LAMP-1) (Flexi-Fluor™)	H4A3	FC
Spark PLUS B550™ anti-human CD107a (LAMP-1) Antibody	H4A3	FC
Spark Red™ 718 anti-human CD107a (LAMP-1) Antibody	H4A3	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07-22-2022

For Research Use Only. Not for diagnostic or therapeutic use.

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