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Purified anti-human CD244 (2B4) Antibody

Pricing & Availability
Clone
2-69 (See other available formats)
Regulatory Status
RUO
Other Names
2B4, NAIL, SLAMF4
Isotype
Mouse IgG2a, κ
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Product Citations
publications
2-69_PURE_CD244_Antibody_030818
Human lysed whole blood stained with CD56 APC and purified CD244 (clone 2-69, top) or mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a FITC.
  • 2-69_PURE_CD244_Antibody_030818
    Human lysed whole blood stained with CD56 APC and purified CD244 (clone 2-69, top) or mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a FITC.
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393502 100 µg 99€
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Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Cyt - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: redirected killing assays (rADCC) in NK cells1.

Application References
  1. Nakajima H, et al. 2000. Eur J Immunol. 30:3309. (Cytotoxicity, IP)
  2. Tassi I, Colonna M. 2005. J. Immunol. 7996-8002. (IP)
Product Citations
  1. van der Sluis TC, et al. 2023. Cell Rep Med. 100939:4. PubMed
RRID
AB_2728427 (BioLegend Cat. No. 393502)

Antigen Details

Structure
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution

NK, T subset, monocytes, basophils, and eosinophils

Function
Activation and inhibition of NK cell function
Ligand/Receptor
CD48
Cell Type
Basophils, Eosinophils, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Lee KM, et al. 2003. J. Immunol. 170:4881.
  2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159.
  3. Messmer B, et al. 2006. J. Immunol. 176:4646.
  4. Boles KS, et al. 2001. Immunol. Rev. 181:234.
Gene ID
51744 View all products for this Gene ID
UniProt
View information about CD244 on UniProt.org
Go To Top Version: 1    Revision Date: 03-12-2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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