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Purified anti-human CD271 (p75NTR) Antibody (Previously Covance catalog# MMS-5228)

Pricing & Availability
Clone
NGFR5 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor receptor superfamily member 16, p75 ICD, NGF receptor, TNFR superfamily, member 16, low affinity nerve growth factor receptor, low affinity neurotrophin receptor p75NTR, CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR
Previously
Covance Catalog# MMS-5228
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
NGFR5_Purified_CD271_p75NTR_Antibody_IHC_030415
IHC staining of purified anti-human CD271 (p75NTR) antibody (clone NGFR5) on formalin-fixed paraffin-embedded normal human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • NGFR5_Purified_CD271_p75NTR_Antibody_IHC_030415
    IHC staining of purified anti-human CD271 (p75NTR) antibody (clone NGFR5) on formalin-fixed paraffin-embedded normal human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • NGFR5_Purified_CD271_p75NTR_Antibody_IHC_070318
    IHC staining of purified anti-human CD271 (p75NTR) antibody (clone NGFR5) on formalin-fixed paraffin-embedded normal human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
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818201 100 µL 220€
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Description

CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis and to regulate cell differentiation and neurogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against A875 human myeloma cells and p75NTR purified from A875 human myeloma cells.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564818 (BioLegend Cat. No. 818201)

Antigen Details

Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
4804 View all products for this Gene ID
UniProt
View information about CD271 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 07-09-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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