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Purified anti-human CD368 (CLEC4D) Antibody

Pricing & Availability
Clone
9B9 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
C-type lectin domain family 4 member D, CLEC6, CLECSF8, MCL, MPCL, Dectin-3, CD368
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
9B9_PURE_CD368_Antibody_1_FC_042016
Human peripheral blood granulocytes were stained with purified anti-human CD368 (CLEC4D, clone 9B9), PE filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 9B9_PURE_CD368_Antibody_1_FC_042016
    Human peripheral blood granulocytes were stained with purified anti-human CD368 (CLEC4D, clone 9B9), PE filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 9B9_PURE_CD368_Antibody_2_ICC_080819
    HEK293 transiently transfected with CLEC4D-FLAG and FcRg chain cells were fixed with 2% paraformaldehyde (PFA) for twenty minutes and blocked/permeabilized with 10% FCS/0.05% saponin in PBS for one hour. Then the cells were stained with 5 µg/ml purified anti-human CD368 (clone 9B9) in 2% FCS/0.05% saponin in PBS for one hour at room temperature, followed by 2 µg/ml Alexa Fluor® 488 rabbit anti-mouse IgG (green) in 2% FCS/0.05% saponin for ten minutes at room temperature. Nuclei were counterstained with DRAQ5™ (blue). The image was captured with a 63X objective. Image provided by Jenny Ostrop of the Norwegian University of Science and Technology.
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360202 100 µg 212€
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Description

CD368 (CLEC4D), also known as CLEC6, is a 30 kD single pass type II transmembrane protein and member of the C-type lectin-like domain (CTLD) superfamily. CLEC4D is an endocytic receptor, probably involved in antigen presentation, cell adhesion, and cell signaling. CLEC4D is expressed by macrophages, monocytes, neutrophils, and a subset of dendritic cells and is upregulated by IL-6, IL-10, TNF-α, and IFN-γ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CLEC4D ectodomain:IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562508 (BioLegend Cat. No. 360202)

Antigen Details

Structure
Member of the C-type lectin-like domain (CTLD) superfamily, single pass type II transmembrane protein, 30 kD
Distribution

Macrophages, monocytes, neutrophils, subset of dendritic cells; low expression in peripheral blood lymphocytes

Function
Endocytic receptor, probably involved in antigen presentation, cell adhesion, and cell signaling
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Zhu LL, et al. 2013. Immunity 39:11.
2. Graham LM, et al. 2012. J. Biol. Chem. 287:25964.
3. Arce I, et al. 2004. Eur. J. Immunol. 34:210.

Gene ID
338339 View all products for this Gene ID
UniProt
View information about CD368 on UniProt.org
Go To Top Version: 5    Revision Date: 08-08-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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