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Purified anti-human CD90 (Thy1) Antibody

Pricing & Availability
Clone
S22015F (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_S22015F_PURE_CD90_Antibody_012624
HEL cells (Human erythroleukemia cell line) were stained with purified anti-human CD90 (Thy1) (clone S22015F) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • A_S22015F_PURE_CD90_Antibody_012624
    HEL cells (Human erythroleukemia cell line) were stained with purified anti-human CD90 (Thy1) (clone S22015F) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • B_S22015F_PURE_CD90_Antibody_IHC_012624
    IHC staining of purified anti-human CD90 (Thy1) (clone S22015F) on formalin-fixed paraffin-embedded human kidney (A) or tonsil (B, negative control) tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissues were incubated with 10 μg/mL of purified anti-human CD90 (Thy1) (clone S22015F) at 4°C overnight, followed by incubation with 2.5 μg/mL of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective.
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389902 100 µg 136€
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Description

CD90, also known as Thy-1, is a 25-35 kD GPI-anchored protein. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD90-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S22015F does not block the binding of clones S22015B nor 5E10 on target cells.

RRID
AB_3106022 (BioLegend Cat. No. 389902)

Antigen Details

Structure
25-35 kD glycoprotein, Ig superfamily
Distribution

Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons, and some leukemia cell lines

Function
Cell adhesion
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Neurons, Thymocytes
Biology Area
Cell Adhesion, Neurodegeneration, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. McKenzie JL, et al. 1981. J Immunol. 126:843-50.
  2. Avalos AM, et al. 2002. Biol Res. 35:231-8.
  3. Wetzel A, et al. 2004. J Immunol. 172:3850-9.
Gene ID
7070 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org
Go To Top Version: 1    Revision Date: 01-26-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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