Purified anti-human CD95 Fas Maxpar Ready Antibody anti-CD95

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Clone: DX2 (See other available formats)
Regulatory Status: RUO
Workshop: VI C-64
Other Names: Fas, APO-1, TNFRSF6
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 164Dγ-anti-CD95 [FAS] (DX2). Data provided by DVS Sciences.

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305631

100 µg

169€

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Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD95 transfected L cells
Formulation: Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis³ (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining^4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563766 (BioLegend Cat. No. 305631)

Antigen Details

Structure: TNFR superfamily, type I transmembrane protein, 45 kD
Distribution: T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
Function: Mediates apoptosis
Ligand/Receptor: Fas ligand (CD178)
Cell Type: B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
Gene ID: 355 View all products for this Gene ID
UniProt: View information about CD95 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD95 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD95 (Fas)	DX2	FC
Biotin anti-human CD95 (Fas)	DX2	FC
FITC anti-human CD95 (Fas)	DX2	FC
PE anti-human CD95 (Fas)	DX2	FC
PE/Cyanine5 anti-human CD95 (Fas)	DX2	FC
Purified anti-human CD95 (Fas)	DX2	FC,ICC,IHC
Alexa Fluor® 488 anti-human CD95 (Fas)	DX2	FC
Alexa Fluor® 647 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 421™ anti-human CD95 (Fas)	DX2	FC
Pacific Blue™ anti-human CD95 (Fas)	DX2	FC
PE/Cyanine7 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 605™ anti-human CD95 (Fas)	DX2	FC
PerCP/Cyanine5.5 anti-human CD95 (Fas)	DX2	FC
Purified anti-human CD95 (Fas) (Maxpar® Ready)	DX2	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD95 (Fas)	DX2	FC
APC/Fire™ 750 anti-human CD95 (Fas)	DX2	FC
APC/Cyanine7 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 510™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 711™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 785™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 650™ anti-human CD95 (Fas)	DX2	FC
Alexa Fluor® 700 anti-human CD95 (Fas)	DX2	FC
TotalSeq™-A0156 anti-human CD95 (Fas)	DX2	PG
TotalSeq™-C0156 anti-human CD95 (Fas)	DX2	PG
TotalSeq™-B0156 anti-human CD95 (Fas)	DX2	PG
Ultra-LEAF™ Purified anti-human CD95 (Fas)	DX2	FC,ICC,IHC,Apop
TotalSeq™-D0156 anti-human CD95 (Fas)	DX2	PG
PE/Fire™ 640 anti-human CD95 (Fas)	DX2	FC
KIRAVIA Blue 520™ anti-human CD95 (Fas)	DX2	FC
APC/Fire™ 810 anti-human CD95 (Fas) Antibody	DX2	FC
PE anti-human CD95	DX2	FC
Spark YG™ 593 anti-human CD95 (Fas)	DX2	FC
PE/Fire™ 700 anti-human CD95 (Fas)	DX2	FC
PerCP/Fire™ 780 anti-human CD95 (Fas)	DX2	FC
Spark Blue™ 574 anti-human CD95 (Fas)	DX2	FC
Spark Red™ 718 anti-human CD95 (Fas)	DX2	FC
GMP PE anti-human CD95 (Fas)	DX2	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07-22-2022

For Research Use Only. Not for diagnostic or therapeutic use.

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