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Purified anti-human Granzyme A Antibody

Pricing & Availability
Clone
W19350D (See other available formats)
Regulatory Status
RUO
Other Names
Hanukah factor serine protease (HFSP), Cytolytic T cell-and natural killer cellspecific trypsin-like serine protease, Cytotoxic T-lymphocyte-associated serineesterase 3 (CTLA3)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W19350D_PURE_Granzyme-A_-Direct-ELISA_Antibody_052323
Direct ELISA showing purified anti-human Granzyme A (clone W19350D) bound to plate-immobilized recombinant human Granzyme A protein (Cat. Nos. 550802 and 550804). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-mouse IgG (Cat. No. 405306). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W19350D_PURE_Granzyme-A_-Direct-ELISA_Antibody_052323
    Direct ELISA showing purified anti-human Granzyme A (clone W19350D) bound to plate-immobilized recombinant human Granzyme A protein (Cat. Nos. 550802 and 550804). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-mouse IgG (Cat. No. 405306). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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617252 100 µg 212€
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Description

Granzyme A is a serine protease belonging to the granzyme family and is expressed exclusively by cytotoxic T cells (CTL) and NK cells. Most circulating CD56+CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzymes A and B. In contrast, few circulating CD4+ T cells express granzyme A or B. Activation of CD8+ and CD4+ T lymphocytes induces substantial expression of granzyme B, but not granzyme A. Following receptor-mediated conjugate formation between a granzyme-containing cell and an infected or transformed target cell, granzymes enter the target cell via endocytosis and induce apoptosis. Granzyme A was found to induce caspase-independent cell death when it enters into the target cell by perforin. Once in a cell, granzyme A activates DNA nicking by DNAse NM23-H1, a tumor suppressor gene product whose expression is reduced in transformed, metastatic cells. Dysregulation of this pathway results in several human diseases, such as hemophagocytic lymphohistiocytosis. Besides the protease activity, granzyme A induces human lung fibroblasts to produce IL-6 and IL-8. Cytokine induction is abrogated by treating the serine protease with the suicide serine protease inhibitor 3,4-dichloroisocoumarin. Other fibroblast lines, as well as epithelial cells, produce cytokines in response to granzyme A. These findings suggest that granzyme A can function as an activation molecule with potentially important immunoregulatory functions. However, CTLs from mice lacking granzyme A induce morphologically normal apoptosis in vitro, but those from mice that are deficient of granzyme B induce the nuclear features of apoptosis (particularly DNA fragmentation) more slowly than do wild-type CTLs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant Granzyme A Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested 
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19350D) could function as a detection antibody when paired with purified anti-human Granzyme A antibody (Cat No. 617152) (clone W19350A) as the capture antibody and using Recombinant Human Granzyme A protein (Cat. Nos. 550802 and 550804) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_3097179 (BioLegend Cat. No. 617252)

Antigen Details

Structure
Monomer
Distribution

CTL and NK cells

Function
Granzyme A is able to induce target cell apoptosis by activating caspase independent pathways.
Interaction
Targets of CTL and NK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Edwards KM, et al. 1999. J Biol Chem. 274:30468-73.
  2. Lieberman J and Fan Z. 2003. Curr Opin Immunol. 15:553-9.
  3. Fan Z, et al. 2003. Cell. 112:659-72.
  4. Sower LE, et al. 1996. Cell Immunol. 171:159-63.
  5. Grossman WJ. et al. 2004. Blood. 104:2840-8.
  6. Ebnet K, et al. 1995. EMBO J. 14:4230-9.
  7. Heusel JW, et al. 1994. Cell. 76:977-87.
Gene ID
3001 View all products for this Gene ID
UniProt
View information about Granzyme A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06-14-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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