Purified anti-human Keratan Sulfate (KS) Antibody

Pricing & Availability
Clone
R-10G (See other available formats)
Regulatory Status
RUO
Other Names
KS, Keratan Sulfate, Keratosulfate
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
R-10G_PURE_Keratan-Sulfate_Antibody_1_091019.png
Human teratocarcinoma cell line, NCCIT, stained with purified anti-human keratan sulfate (clone R-10G, filled histogram) or purified Mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • R-10G_PURE_Keratan-Sulfate_Antibody_1_091019.png
    Human teratocarcinoma cell line, NCCIT, stained with purified anti-human keratan sulfate (clone R-10G, filled histogram) or purified Mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • R-10G_PURE_Keratan-Sulfate_Antibody_2_091019.png
    Human peripheral blood lymphocytes were stained with purified anti-human keratan sulfate (clone R-10G) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE and CD16 FITC.
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397602 100 µg 227€
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Description

Keratan Sulfate (KS) consists of a family of glycosaminoglycans (GAGs) that are abundantly present in the extracellular matrix and cell surface in certain tissues, such as the cornea and cartilage. Clone R-10G recognizes a unique form of podocalyxin-associated KS structures that are enriched on human induced pluripotent stem cells(hiPS) and human embryonic stem cells(hES). The epitope is also expressed in the adult human and mouse brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Tic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814376 (BioLegend Cat. No. 397602)

Antigen Details

Structure
Sulfated glycosaminoglycans, carbohydrate polymer
Distribution

Human iPS/ES cells

Cell Type
Embryonic Stem Cells
Biology Area
Stem Cells
Antigen References
  1. Kawabe K, et al. 2013. Glycobiology. 23:322.
  2. Nakao H, et al. 2017. Glycoconj. J. 34:789.
  3. Shogo M, et al. 2015. jbc.M115.657692.
  4. Makanga JO, et al. 2015. Biol Pharm Bull. 38:127
  5. Hiroaki T, et al. 2017. Regen Ther. 6:1-8
Gene ID
NA
UniProt
View information about Keratan Sulfate on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09-10-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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