Purified anti-Leptin Antibody

Pricing & Availability
Clone
M1905E01 (See other available formats)
Regulatory Status
RUO
Other Names
Obese Protein, Obesity Factor, LEPD, OB, OBS
Isotype
Mouse IgG2a, λ
Ave. Rating
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Product Citations
publications
M1905E01_Purified_Leptin_Antibody_WB_022715
Western blot analysis of recombinant human leptin 25 ng (lane 1), and 50 ng (lane 2) using anti-Leptin antibody (clone M1905E01) and HRP anti-mouse IgG antibody.
  • M1905E01_Purified_Leptin_Antibody_WB_022715
    Western blot analysis of recombinant human leptin 25 ng (lane 1), and 50 ng (lane 2) using anti-Leptin antibody (clone M1905E01) and HRP anti-mouse IgG antibody.
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675002 100 µg 212€
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Description

Leptin is primarily synthesized from adipocytes and plays a major role in energy homeostasis and obesity. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, expression of the short form of the leptin receptor, Ob-Ra, is downregulated, and the individual may be unresponsive to leptin signals. Leptin has also been implicated in the regulation of reproduction, glucose homeostasis, bone formation, hematopoiesis, and wound healing.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Leptin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Lee S, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2565487 (BioLegend Cat. No. 675002)

Antigen Details

Structure
167 amino acids with a predicted molecular weight of approximately 18 kD.
Distribution

Secreted by adipocytes, gastric epithelial cells, or fibroblasts.

Function
Plays a major role in energy homeostasis and obesity.
Interaction
Interacts with Siglec-6.
Ligand/Receptor
Leptin receptors.
Cell Type
Epithelial cells, Fibroblasts
Biology Area
Angiogenesis, Cell Biology, Signal Transduction
Molecular Family
Enzymes and Regulators
Antigen References

1. Ando S, et al. 2014. Front Oncol. 4:340.
2. Upadhyay J, et al. 2015. Metabolism 64:105.
3. Sainz N, et al. 2015. Metabolism 64:35.
4. Zhang F, et al. 1997. Nature 387:206.
5. Brennan AM, et al. 2006. Nat. Clin. Pract. Endocrinol. Metab. 2:318.
6. Halaas JL, et al. 1995. Science 269:543.

Gene ID
3952 View all products for this Gene ID
UniProt
View information about Leptin on UniProt.org
Go To Top Version: 2    Revision Date: 06-14-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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