- Clone
- W18113C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Malic Enzyme 2; NAD-Dependent Malic Enzyme, Mitochondrial; Malate Dehydrogenase (Oxaloacetate-Decarboxylating); Malic Enzyme 2, NAD(+)-Dependent, Mitochondrial; NAD-ME; Pyruvic-Malic Carboxylase
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
635051 | 25 µg | 112€ | ||||
635052 | 100 µg | 316€ |
ME2, also known as malic enzyme 2, is a mitochondrial NAD- or NADP-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, involving the tricarboxylic acid (TCA) cycle for energy production. During the conversion of malate to pyruvate, ME2 also reduces NAD+ to NADH, participating in cellular respiration and serving as an electron carrier in the electron transport chain. It is a heteromeric protein that plays a crucial role in energy metabolism, cancer, cardiovascular disease, and neurological disorders. ME2 has been found to be upregulated in various types of cancer, including breast, lung, and liver cancer, promoting tumor growth and survival by providing cancer cells with the necessary energy and building blocks for rapid proliferation. Additionally, ME2 assists in glutaminolysis, providing another energy source for cancer cell proliferation. Moreover, P53 has been found to lower the expression of ME2, which induces ROS levels and decreases ATP production, preventing cellular senescence of tumor cells. Furthermore, there may be a link between ME2 and family-associated genetic generalized epilepsy.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human ME2 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W18113C is not recommended for WB and IHC-P.
For Immunocytochemistry (ICC), ice-cold methanol is recommended for the fixation and permeabilization method. - RRID
-
AB_3106145 (BioLegend Cat. No. 635051)
AB_3106145 (BioLegend Cat. No. 635052)
Antigen Details
- Structure
- ME2 has two isoforms due to alternative splicing.
- Distribution
-
Wide tissue expression/mitochondrion matrix
- Function
- Catalyzes the oxidative decarboxylation of malate to pyruvate/regulate TCA cycle
- Interaction
- Form homodimer and homotetramer
- Cell Type
- Leukemia, Neurons, Osteoblasts
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Proliferation and Viability, Mitochondrial Function, Neuroscience, Synaptic Biology
- Molecular Family
- Enzymes and Regulators, Mitochondrial Markers, Organelle Markers
- Antigen References
-
- Pongratz R, et al. 2007. J Biol Chem. 282:200-7.
- MacDonald M, et al. 2009. Arch Biochem Biophys. 488:100-4.
- Wen Y, et al. 2014. Acta Pharmacol Sin. 35:674-84.
- Ren J, et al. 2014. Sci Rep. 4:5414.
- Wang Y, et al. 2021. Cell Metab. 33:1027-1041.
- Hsieh J, et al. 2023. Commun Biol. 6:548.
- Li W, et al. 2023. Proc Natl Acad Sci U S A. 120:e2217869120
- Gene ID
- 4200 View all products for this Gene ID
- UniProt
- View information about ME2 on UniProt.org
Related FAQs
Other Formats
View All ME2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ME2 | W18113C | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-ME2
A-431 cells were stained with 500 nM of MitoSpy™ Green FM (C...
Follow Us