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Purified anti-mouse CD319 Antibody

Pricing & Availability
Clone
4G2 (See other available formats)
Regulatory Status
RUO
Other Names
19A, CS1, CD319, CRACC, SLAMF7
Isotype
Rat IgG1, κ
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Product Citations
publications
4G2_PURE_CD319_Antibody_1_060616
C57BL/6 mouse splenocytes were stained with purified CD319 (Clone 4G2, top) or purified rat IgG1, κ isotype control (bottom), followed by anti-rat IgG PE and then anti-mouse CD19 FITC.
  • 4G2_PURE_CD319_Antibody_1_060616
    C57BL/6 mouse splenocytes were stained with purified CD319 (Clone 4G2, top) or purified rat IgG1, κ isotype control (bottom), followed by anti-rat IgG PE and then anti-mouse CD19 FITC.
  • 4G2_PURE_CD319_Antibody_2_060616
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152002 100 µg 231€
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Description

CD319/CD319/CS1/CRACC/SLAMF7 is a 66 kD transmembrane protein belonging to the CD2 subset of the immunoglobulin superfamily; it is expressed on most NK cells, B cells, and subsets of T cells. CD319 activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT) and inhibits immune responses in the absence of SAP-family adaptors or when bound to SH-domain molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD319-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Direct ELISA, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616915 (BioLegend Cat. No. 152002)

Antigen Details

Structure
Extracellular domain with one Ig like V-set domain and one Ig like C2-set domain, and an cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs.
Distribution

Spleen, lymph node, bone marrow, testis, thymus; NK cells, B cells, and subsets of T cells.

Function
Activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT).
Interaction
Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN, and SAP-family adaptors (SAP, EAT-2, ERT).
Cell Type
B cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Li Y, et al. 2013. PLoS One 8(9):e76681.
2. Dongre P, et al. 2013. Mol. Immunol. 54:254-63.
3. Tovar V, et al. 2002. Immunogenetics 54:394.
4. Dong Z, et al. 2009. Nat. Immunol. 10:973.

Gene ID
75345 View all products for this Gene ID
UniProt
View information about CD319 on UniProt.org
Go To Top Version: 2    Revision Date: 10-05-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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