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Purified anti-mouse H-2 Antibody

Pricing & Availability
Clone
M1/42 (See other available formats)
Regulatory Status
RUO
Other Names
Mouse major histocompatibility complex (MHC) H-2
Isotype
Rat IgG2a, κ
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Product Citations
publications
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125502 100 µg 165€
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Description

The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References
  1. Stallcup KC, et al. 1981. J. Immunol. 127:923.
  2. Thor G, et al. 1993. J. Immunol. 151:211. (IP)
  3. Arendt C and Ostergaard H. 1995. J. Biol. Chem. 270:2313. (IP)
Product Citations
  1. Pilat N, et al. 2016. JCI Insight. e85911:1. PubMed
  2. Nakanishi Y, et al. 2018. Immunity. 49:1132. PubMed
RRID
AB_1227704 (BioLegend Cat. No. 125502)

Antigen Details

Structure
45kd heavy chain non-covalently associated with 12 kd beta-2 microglobulin
Distribution
All nucleated cells
Function
Present antigenic peptide to CD8+ cytotoxic T cells
Ligand/Receptor
MHC/peptide
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Stallcup KC, et al. 1981. J. Immunol. 127:923.

Gene ID
111364 View all products for this Gene ID
UniProt
View information about H-2 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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