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Purified anti-mouse P2RY12 Antibody

Pricing & Availability
Clone
S16007D (See other available formats)
Regulatory Status
RUO
Other Names
P2Y12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled, 12
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
S16007D_PURE_P2RY12_Antibody_2_032618
IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • S16007D_PURE_P2RY12_Antibody_2_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • S16007D_PURE_P2RY12_Antibody_3_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • S16007D_PURE_P2RY12_Antibody_4_032618
    Single cell suspension of mouse brain tissue was stained with purified anti-P2RY12 antibody (clone S16007D, blue histogram) or rat IgG isotype control (red histogram), followed by anti-rat antibody conjugated to APC for detection.
  • S16007D_PURE_P2RY12_Antibody_6_032618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on 4%-formaldehyde fixed mouse brain tissue. Following thick (100µm) vibratome sectioning, the slices were blocked for 1h at RT with 10%NGS and 1%BSA in PBST (pH=7.4 PBS + 0.1% Tween20), incubated with the primary antibody at 5 µg/ml overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 2h at RT. CX3CR1-GFP signal enabled validation of the microglial-specific anti-P2RY12 staining (pseudocolored red). The above images are maximal intensity projections from 40µm-thick z-stacks captured with a 60X objective on a confocal laser scanning microscope. The images are a courtesy of Dr. Alberto Sepulveda-Rodriguez and Dr. Stefano Vicini, Department of Pharmacology and Physiology, Interdisciplinary Program in Neuroscience, Georgetown University Medical Center.
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848001 25 µg 90€
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848002 100 µg 235€
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Description

Purinergic Receptor P2Y (P2RY12) is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. P2RY12 is not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. P2RY12 is a highly selective marker for microglia that specifically distinguishes these cells from other myeloid cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse P2RY12.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For flow cytometric staining. a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research.

Learn more about the validation testing we performed on clone S16007D by watching our Scientific Poster Video.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. He D, et al. 2022. Immunity. 55:159. PubMed
  2. Zhao Q, et al. 2022. Proc Natl Acad Sci U S A. 119:e2113310119. PubMed
  3. Chen S, et al. 2022. Acta Neuropathol Commun. 10:188. PubMed
  4. Salvany S, et al. 2022. Brain Pathol. 32:e13078. PubMed
  5. Albertini G, et al. 2023. Mol Psychiatry. . PubMed
  6. Gras S, et al. 2021. Aging (Albany NY). 13:18051. PubMed
  7. Manich G, et al. 2020. Front Cell Neurosci. 14:567404. PubMed
  8. Malsy J, et al. 2020. Elife. 9:00. PubMed
  9. Chen HR, et al. 2022. Theranostics. 12:512. PubMed
  10. Werneburg S, et al. 2020. Immunity. 52(1):167-182.e7.. PubMed
  11. Shi X, et al. 2021. Nat Commun. 12:6943. PubMed
  12. Safaiyan S, et al. 2021. Neuron. 109(7):1100-1117.e10. PubMed
  13. Zhou X, et al. 2020. Nat Neurosci. 1.192361111. PubMed
  14. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  15. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
  16. Lin R, et al. 2022. Nat Methods. 19:976. PubMed
  17. Gunner G, et al. 2019. Nat Neurosci. 1.663194444. PubMed
  18. Wang L, et al. 2022. Front Cell Neurosci. 15:786020. PubMed
  19. Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed
  20. de Kleijn KMA, et al. 2022. Biomedicines. 10:. PubMed
  21. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
RRID
AB_2650633 (BioLegend Cat. No. 848001)
AB_2650633 (BioLegend Cat. No. 848002)

Antigen Details

Structure
P2RY12 is a 347 amino acid protein with a molecular mass of 39.8 kD.
Distribution

Tissue distribution: platelets, brain (microglia), lung, appendix, pituitary and adrenal glands.
Cellular distribution: cell membrane and cytosol.

Function
G-protein coupled receptor that inhibits the adenylyl cyclase second messenger system.
Ligand/Receptor
ADP and ATP.
Cell Type
Microglia, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
GPCR, Purinergic Receptors
Antigen References

1. Orr AG, et al. 2009. Nat. Neurosci. 12(7):872-8.

Gene ID
70839 View all products for this Gene ID
UniProt
View information about P2RY12 on UniProt.org
Go To Top Version: 8    Revision Date: 04-28-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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