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Purified anti-MUT Antibody

Pricing & Availability
Clone
W18259A (See other available formats)
Regulatory Status
RUO
Other Names
Methylmalonyl-CoA Mutase, Methylmalonyl Coenzyme A Mutase, MCM, Truncated Methylmalonyl-CoA Mutase Variant, MMUT, EC 5.4.99.2, Methylmalonyl-CoA Isomerase
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18259A_PURE_MUT_Antibody_100720.png
Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) purified anti-MUT antibody (clone W18259A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18259A_PURE_MUT_Antibody_100720.png
    Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) purified anti-MUT antibody (clone W18259A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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936601 25 µg 81€
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936602 100 µg 203€
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Description

MUT encodes MCM (Methylmalonyl-CoA mutase), which resides in the mitochondria to metabolize branched and odd-chain fatty acids. Utilizing cobalamin as a cofactor, MCM also isomerizes methylmalonyl-CoA to make succinyl-CoA, an important intermediate in the Kreb’s Cycle. Mutations in the gene can lead to disruptions in amino acid metabolism and may alter lipid and glucose homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human MUT protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When tested for ICC on 4% PFA-fixed HeLa cells, this clone produced punctate nuclear staining, which is inconsistent with the reported mitochondrial matrix localization of MUT. We therefore do not recommend this clone for the application.

RRID
AB_2888896 (BioLegend Cat. No. 936601)
AB_2888896 (BioLegend Cat. No. 936602)

Antigen Details

Structure
MUT is a 750 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

MUT expression is localized to the cytosol and the mitochondria. 

Function
Fatty-acid metabolism; isomerase
Interaction
Adenosylcobalamin (Adocbl)
Antigen References

1. Janata J, et al. 1997.  Hum. Mol. Gen. 6(9):1457-64.
2. Worgan LC, et al. 2006. Hum. Mutat. 27(1):31-43.
3. Mancia F, et al. 1998. Cell. 6(6):711-20.
4. Reid M, et al. 2017. Cell. 171(4):736-37.
5. Schneller J, et al. 2015.  Mol. Ther. 23(1):70.

Gene ID
4594 View all products for this Gene ID
UniProt
View information about MUT on UniProt.org
Go To Top Version: 1    Revision Date: 10-07-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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