- Clone
- F5D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Myogenin (MYOG), Class C basic helix-loop-helix protein 3 (BHLHC3), Myogenic factor 4 (Myf-4)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
608851 | 25 µg | 124€ | ||||
608852 | 100 µg | 278€ |
Myogenin is a basic helix-loop-helix transcription factor from the myogenic regulator factor (MRF) family of transcription factors, which also includes MyoD, Myf5 and MRF4. Like other members of the MRF family myogenin is required for skeletal muscle development, and regulates the growth, repair and atrophy of skeletal muscle and myosatellite cells. In mice, loss of myogenin expression results in perinatal lethality resulting from major deficits in skeletal muscle formation. In humans, myogenin has diagnostic value as a marker for rhabdomyosarcoma and other related cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide containing a fragment of the N-terminus of rat myogenin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product was tested for use in immunocytochemistry and flow cytometry in HeLa and RD cells but did not meet passing criteria.
This product was tested for use in IHC-P in human and mouse skeletal muscle tissue but did not meet passing criteria.
- RRID
-
AB_2927974 (BioLegend Cat. No. 608851)
AB_2927974 (BioLegend Cat. No. 608852)
Antigen Details
- Structure
- Myogenin is a 224 amino-acid protein with a predicted molecular weight of 25.0 kd.
- Distribution
-
Myogenin is expressed in the nucleus of skeletal muscle and myosatellite cells.
- Function
- Transcription factor
- Interaction
- Myogenin interacts with MDFI, POLR2C, SRF, SP1, and TCF3
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
-
- Boyer J, et al. 2014. Hum Mol Genet. 23:4249-59.
- Nabeshima Y, et al. 1993. Nature. 346:532-5.
- Zammit P. 2017. Semin Cell Dev Biol. 72:19-32
- Gene ID
- 4656 View all products for this Gene ID
- UniProt
- View information about Myogenin on UniProt.org
Related FAQs
Other Formats
View All Myogenin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Myogenin | F5D | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Myogenin
Whole cell extracts (15 µg total protein) from the indicated...
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