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Purified anti-PARIS (ZNF746) Antibody (Previously Covance catalog# MMS-5178)

Pricing & Availability
Clone
N196/16 (See other available formats)
Regulatory Status
RUO
Other Names
Zinc finger protein 746, parkin-interacting sustrate, parkin-interacting substrate, ZNF746
Previously
Covance Catalog# MMS-5178
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_N196slash16_PURE_PARIS_IHCP_Mouse_050418
IHC staining of purified anti-PARIS (ZNF746) antibody (clone N196/16) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_N196slash16_PURE_PARIS_IHCP_Mouse_050418
    IHC staining of purified anti-PARIS (ZNF746) antibody (clone N196/16) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_N196slash16_PURE_PARIS_IHCP_Rat_050418
    IHC staining of purified anti-PARIS (ZNF746) antibody (clone N196/16) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_N196slash16_PURE_PARIS_WB_MouseRat_050418
    Western blot of purified anti-PARIS (ZNF746) antibody (clone N196/16). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • D_N196slash16_PURE_PARIS_WB_Human_050418
    Western blot of purified anti-PARIS (ZNF746) antibody (clone N196/16). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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820601 100 µL 132€
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Description

Parkin-interacting substrate (PARIS), also known as zing finger protein 746 (ZNF746), accumulates in human Parkinson's disease brain. PARIS levels are controlled by parkin, and dysregulation of parkin leads to PARIS-dependent loss of dopaminergic neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-322 (N-terminus) of human PARIS/ZNF746.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

RRID
AB_2564842 (BioLegend Cat. No. 820601)

Antigen Details

Structure
Expected MW: 60 kD
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Gene ID
155061 View all products for this Gene ID
UniProt
View information about PARIS on UniProt.org
Go To Top Version: 5    Revision Date: 07-09-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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