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Purified anti-PAX6 Antibody

Pricing & Availability
Clone
W19167A (See other available formats)
Regulatory Status
RUO
Other Names
Paired Box 6, Paired Box Protein PAX-6, Aniridia type II protein, Oculorhombin, AN2, D11S812E, MGDA, AN, MGC17209, WAGR, Aniridia 1, Aniridia 2, ASGD5, FVH1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19167A_PURE_PAX6_Antibody_1_092820
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-PAX6 antibody (clone W19167A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19167A_PURE_PAX6_Antibody_1_092820
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-PAX6 antibody (clone W19167A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19167A_PURE_PAX6_Antibody_2_092820.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-PAX6 antibody (clone W19167A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-PAX6 antibody (clone AD2.35) (Cat. No. 862002). Lane M: Molecular weight marker.
  • W19167A_PURE_PAX6_Antibody_3_080621
    IHC staining of purified anti-PAX6 antibody (clone W19167A) on frozen cerebellum brain (A, positive control) and sciatic nerve (B, negative control) tissues. Following fixation (Cat. No. 420801) and permeabilization with 0.5% Triton X-100, the tissue sections were incubated with 0.5 µg/mL of purified anti-PAX6 antibody overnight at 4°C. Alexa Fluor® 594 anti-rat IgG2a antibody (Cat. No. 407509) was used for 2-hour incubation at room temperature, followed by nuclei counterstain with DAPI (Cat. No. 422801). The slides were then mounted with ProLong™ Gold Antifade Mountant for image acquisition with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Check Availability Save
939801 25 µg 95€
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939802 100 µg 235€
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Description

Paired box protein 6 (PAX6) is a transcription factor with dual roles as both transcriptional activator and repressor. It plays a critical role in the regulation of cell proliferation, cell cycle length and exit, and cell differentiation during neurogenesis and ocular, cortical, and olfactory development. PAX6 forms a complex with SOX2 and δ-crystallin enhancer DC5 to initiate lens placode development. In the central nervous system, PAX6 functions in the regulation of progenitor cell proliferation, telencephalic patterning, cortical layer formation, and the maintenance of balance in neuron differentiation.   Heterozygous mutations in PAX6 can result in aniridia, nystagmus, neurodevelopmental disorders, and auditory and memory impairment. In the neuroendocrine system, PAX6 functions in the development of β cells, pancreatic islet of Langerhans cells, and the pituitary gland as well as in insulin biosynthesis, and glucose-induced insulin secretion. PAX6 functions as a tumor suppressor in prostate cancer and glioblastoma by inhibiting androgen receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to C-terminus of human PAX6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.25 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunohistochemistry on frozen tissue sections, a concentration range of 0.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

At higher concentrations, this clone recognizes a ~100 kD protein of unknown origin. This protein was not detected in W19167A immunoprecipitates that were probed by western blot using W19167A.

RRID
AB_2888904 (BioLegend Cat. No. 939801)
AB_2888904 (BioLegend Cat. No. 939802)

Antigen Details

Structure
PAX6 is a 422 amino acid protein with a predicted molecular weight of 47 kD.
Distribution

Expressed in the eye, nose, brain pancreas/Nucleoplasm

Function
Transcriptional regulation, differentiation
Antigen References

1. Gosmain Y, B, et al. 2012. Mol.. Endocrinol. 26:696
2. Kamachi Y, et al. 2001. Genes Dev. 10:1272
3. Manuel M, et al. 2015. Fron. Cell. Neurosci. 9:70
4. Shyr C, et al. 2010. Prostate. 274:6056

Gene ID
5080 View all products for this Gene ID
UniProt
View information about PAX6 on UniProt.org
Go To Top Version: 2    Revision Date: 08-06-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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