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Purified anti-PIEZO1 Antibody

Pricing & Availability
Clone
W17241A (See other available formats)
Regulatory Status
RUO
Other Names
PIEZO Type Mechanosensitive Ion Channel Component 1, Membrane Protein Induced By Beta-Amyloid Treatment, FAM38A, Mib, KIAA023, LMPH3, DHS
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_W17241A_PURE_PIEZO1_Antibody_3_011222
IHC staining of purified anti-PIEZO1 (clone W17241A) on human bladder. Tissue was fixed with Fixation Buffer (Cat. No. 420801), blocked with 5% FBS + 5% Normal Blocking Serum (Cat. No. 927503), and incubated with 10.0 µg/mL purified rat IgG2b, κ isotype control (Cat. No. 400602) (panel A) or 10.0 µg/mL of anti-PIEZO1 antibody (panel B) overnight at 4°C. This was followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
  • 1_W17241A_PURE_PIEZO1_Antibody_3_011222
    IHC staining of purified anti-PIEZO1 (clone W17241A) on human bladder. Tissue was fixed with Fixation Buffer (Cat. No. 420801), blocked with 5% FBS + 5% Normal Blocking Serum (Cat. No. 927503), and incubated with 10.0 µg/mL purified rat IgG2b, κ isotype control (Cat. No. 400602) (panel A) or 10.0 µg/mL of anti-PIEZO1 antibody (panel B) overnight at 4°C. This was followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
  • 2_W17241A_PURE_PIEZO1_Antibody_2_011222
    Whole cell lysates from HeLa (15 μg) untreated or treated with non-targeting siRNA (siCON) or siRNA targeting PIEZO1 (siPIEZO1) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 μg/mL (1:500 dilution) of purified anti-PIEZO1 (clone W17241A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
  • 3_W17241A_PURE_PIEZO1_Antibody_1_011222
    Whole cell extracts (15 µg total protein) from HeLa, A-431, and PC-3 cells were heated to 37°C for 30 minutes and resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-PIEZO1 (clone W17241A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker
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602751 25 µg 95€
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602752 100 µg 235€
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Description

PIEZO1 and its paralog PIEZO2 are members of a non-selective cationic mechanosensitive channel family first discovered in a neuronal cell line through mechanical stimulation. It responds to mechanical forces such as static pressure, shear stress, and membrane stretch events by allowing the efflux of calcium ions from the ER lumen into the cytosol. The increase in cytosolic calcium results in phosphorylation of ERK1/2 and a commensurate induction of downstream mitotic genes, and can also promote prostate cancer development via activation of the Akt/mTOR signaling axis. PIEZO1 is overexpressed in many tumors and is a key regulator of tumor stiffness.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PIEZO1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When testing this clone for western blot, we observed a considerable loss of signal with lysates that were boiled in sample buffer compared to samples that were heated to 37°C. This is likely due to high temperatures causing aggregation of PIEZO1, which is a multi-pass transmembrane domain protein.

This clone was tested for immunocytochemistry on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in high non-specific staining.

RRID
AB_2910478 (BioLegend Cat. No. 602751)
AB_2910478 (BioLegend Cat. No. 602752)

Antigen Details

Structure
PIEZO1 is a 2521 amino acid protein with a predicted molecular weight of 287 kD.
Distribution

Ubiquitously expressed/Endoplasmic reticulum

Function
Mechanically-activated ion channel
Cell Type
Epithelial cells
Biology Area
Neuroscience, Signal Transduction
Molecular Family
Ion Channels
Antigen References
  1. Gudipaty SA, et al. 2017. Nature. 543:118-121.
  2. Nonomura K, et al. 2018. PNAS. 115:12817-12822.
  3. Romac JM, et al. 2018. Nat Comm. 9:1715.
  4. Zhao C, et al. 2019. Front Endocrinol. 10:373.
  5. Williams E. 2019. Sci Signal. 12:598.
  6. Han Y, et al. 2019. Int J Onc. 55:629-644.

 

 

Gene ID
9780 View all products for this Gene ID
UniProt
View information about PIEZO1 on UniProt.org
Go To Top Version: 1    Revision Date: 01-12-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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