Purified anti-rat RT1B Antibody

Pricing & Availability
Clone
OX-6 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class II, I-A equivalent
Isotype
Mouse IgG1, κ
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Product Citations
publications
OX-6_Pure_021309.jpg
LOU rat splenocytes stained with anti-rat CD3 (1F4) APC and purified OX-6, followed by anti-mIgG1-FITC
  • OX-6_Pure_021309.jpg
    LOU rat splenocytes stained with anti-rat CD3 (1F4) APC and purified OX-6, followed by anti-mIgG1-FITC
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205301 100 µg 100€
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Description

RT1B is expressed on peripheral B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophages, subsets of thymocytes, peritoneal mast cells, and small intestinal villus epithelium. The OX-6 antibody reacts with non-polymorphic determinants of the rat MHC class II antigen, I-A equivalent. The OX-6 monoclonal antibody cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ia-like Glycoproteins from Wistar Thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Fox CC, et al. 1994. Cell Immunol. 158:253.
  2. Chen-Woan M, et al. 1996. J. Leukocyte Biol. 59:196.
  3. Damoiseaux JG, et al. 1998. J. Leukocyte Biol. 64:803.
  4. De Riva A, et al. 2013. J. Immunol. 190:5961. PubMed
Product Citations
  1. Riva A, et al. 2013. J Immunol. 190:5961. PubMed
  2. Yu Z, et al. 2018. J Transl Med. 0.902083333. PubMed
RRID
AB_1575156 (BioLegend Cat. No. 205301)

Antigen Details

Structure
MHC-class II
Distribution

B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophage, and subsets of thymocytes.

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
B cells, Dendritic cells, Langerhans cells, Macrophages, Thymocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
24738 View all products for this Gene ID
UniProt
View information about RT1B on UniProt.org
Go To Top Version: 3    Revision Date: 09-21-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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