- Clone
- 1Y26 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNA polymerase II sub-unit 3, RPB3, POLR2C
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
665004 | 100 µg | 259€ |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNA polymermase II subunit RPB3 is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft and is known to be involved in transcription termination.
Product DetailsProduct Details
- Verified Reactivity
- Yeast
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- Aliquot and store at -20°C. Avoid freeze/thaw cycles.
- Application
-
WB - Quality tested
ELISA - Verified
ChIP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.
Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation. - Application References
-
- Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
- Product Citations
-
- RRID
-
AB_2565221 (BioLegend Cat. No. 665004)
Antigen Details
- Structure
- RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 854791 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase II RPB3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Polymerase II RPB3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-RNA Polymerase II RPB3 | 1Y26 | WB,ELISA,ChIP |
Direct-Blot™ HRP anti-RNA Polymerase II RPB3 | 1Y26 | WB |
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Compare Data Across All Formats
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Purified anti-RNA Polymerase II RPB3
Western blot analysis of yeast (saccharomyces cerevisiae... -
Direct-Blot™ HRP anti-RNA Polymerase II RPB3
Total cell lysates (15 µg total protein) from HeLa and S. ce...
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