- Clone
- H76 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
625002 | 200 µL | 235€ |
SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- amino acids 216-434 of human SLP-76
- Formulation
-
This antibody is provided in phosphate-buffered solution, containing 0.09% sodium azide.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, aliquot and store frozen at -20°C. Avoid freeze-thaw cycles.
- Application
-
WB - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
The 200 µL size can be used for approximately 40 Western blots.
- Application References
-
- Deford-Watts LM, et al. 2011. J. Immunol. 186:6839. PubMed.
- Product Citations
-
- RRID
-
SCR_001134 (BioLegend Cat. No. 625002)
Antigen Details
- Structure
- Phosphoprotein 76 kD containing SH2 domain in the C-terminus
- Distribution
-
Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.
- Function
- Adaptor molecule involved in B cell receptor and T cell receptor signaling
- Interaction
- Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2
- Modification
- Phosphorylation (Y113, Y128, Y145, Y423, Y426)
- Cell Type
- B cells, Monocytes, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Jackman KJ, et al. 1995. J. Biol. Chem. 270:7029.
2. Wardenberg JB, et al. 1996. J. Biol. Chem. 271:19641.
3. Tuosto L, et al. 1996. J. Exp. Med. 184:1161.
4. Motto DG, et al. 1996. J. Exp. Med. 183:1937. - Gene ID
- 3937 View all products for this Gene ID
- UniProt
- View information about SLP-76 on UniProt.org
Related FAQs
Other Formats
View All SLP-76 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SLP-76 | H76 | WB,IP |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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