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Purified anti-Tau, 1-100 Antibody (Previously Covance catalog# SIG-39402)

Pricing & Availability
Clone
43D (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Previously
Covance Catalog# SIG-39402
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
43D_PURE_Tau1-100_Antibody_WB_051018
Western blot of purified anti-Tau, 1-100 antibody (clone 43D). Lane 1: Molecular weight marker; Lane 2: 20 µg of normal human brain lysate; Lane 3: 20 µg of Alzheimer's disease human brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 43D_PURE_Tau1-100_Antibody_WB_051018
    Western blot of purified anti-Tau, 1-100 antibody (clone 43D). Lane 1: Molecular weight marker; Lane 2: 20 µg of normal human brain lysate; Lane 3: 20 µg of Alzheimer's disease human brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 43D_PURE_Tau1-100_IHC_Antibody_051018
    IHC staining of purified anti-Tau, 1-100 antibody (clone 43D) on formalin-fixed paraffin-embedded Alzheimer's disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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816603 25 µL 132€
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816601 100 µL 444€
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
2 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residues 1-100 of human Tau. The epitope lies within amino acids 6-18. This clone is specific for all six isoforms of human tau protein.

This antibody clone has been reported for use on IHC of 4% PFA-fixed free floating sections5.

This antibody is exclusively provided by BioLegend.

Product Citations
  1. Snellman A, et al. 2022. Brain. 145:2834. PubMed
  2. Gu J, et al. 2020. J Biol Chem. 295:13812. PubMed
  3. Liu F, et al. 2023. Front Aging Neurosci. 15:1073774. PubMed
  4. Wu R, et al. 2021. Front Mol Neurosci. 14:631833. PubMed
  5. Li L, et al. 2021. Acta Neuropathol Commun. 9:28. PubMed
RRID
AB_2564800 (BioLegend Cat. No. 816603)
AB_2564800 (BioLegend Cat. No. 816601)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropath. 103(1):26-35.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.
  3. Dong Y, et al. 2012. PLoS One. 7(6):e39386.
  4. Simic G, et al. 2016. Biomolecules. 6(1):6.
  5. Dai CL, et al. 2017. Alzheimers Res Ther. 9(1):1. (IHC, WB) [PubMed]
  6. Baazaoui N, et al. 2017. Alzheimers Res Ther. 9:45. ( WB) [PubMed]
  7. Dai CL, et al. 2015. J neural Transm (Vienna). 122:607.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau 1-100 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 07-09-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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