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Purified anti-UQCRC1 Antibody

Pricing & Availability
Clone
O91G6 (See other available formats)
Regulatory Status
RUO
Other Names
Cytochrome b-c1 complex subunit 1, mitochondrial, Complex III subunit 1, Core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_O91G6_PURE_UQCRC1_Antibody_WB_1_050417_updated
Total lysates (15µg protein) from Jurkat, HeLa and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-UQCRC1 antibody (clone O91G6, 1 µg/ml) (upper) or GAPDH (poly6314) antibody (lower). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody for UQCRC1 or HRP Donkey anti-rabbit IgG Antibody for GAPDH and chemiluminescence detection. Lane M is the Molecular Weight ladder.
  • 1_O91G6_PURE_UQCRC1_Antibody_WB_1_050417_updated
    Total lysates (15µg protein) from Jurkat, HeLa and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-UQCRC1 antibody (clone O91G6, 1 µg/ml) (upper) or GAPDH (poly6314) antibody (lower). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody for UQCRC1 or HRP Donkey anti-rabbit IgG Antibody for GAPDH and chemiluminescence detection. Lane M is the Molecular Weight ladder.
  • 2_O91G6_PURE_UQCRC1_Antibody_ICC_2_041619
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were stained with DAPI (blue) only (left) or DAPI and intracellular anti-UQCRC1 antibody (right, clone O91G6 at 2 µg/ml) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature.The image was captured with a 60X objective.
  • O91G6_PURE_UQCRC1_Antibody_3_012422
    Total lysates (15 µg protein) from HeLa (Lane 1), 5nM (Lane 2)and 20nM (Lane 3) UQCRC1 siRNA treated HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-UQCRC1 antibody (upper) or GAPDH antibody (lower). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody or HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection. Lane M is the Molecular Weight ladder.
Cat # Size Price Quantity Check Availability Save
697601 25 µg 81€
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697602 100 µg 203€
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Description

The ubiquinol-cytochrome c reductase complex, also called mitochondrial complex III, is the third complex in the mitochondrial electron transport chain. The complex III is composed of 11 subunits that form a multi-segment transmembrane protein. It is part of the mitochondrial respiratory chain and plays essential roles in the synthesis of ATP. UQCRC1 may mediate formation of the complex between cytochromes c and c1.

The dysregulated UQCRC1 is involved in impaired mitochondrial electron transport chain function and was found in clear cell renal cell carcinoma and Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant protein fragment corresponding to amino acids 46-305 of human UQCRC1 expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has very weak cross-reactivity with mouse UQCRC1.

Antibody specificity confirmed by partial knock-down of the target using siRNA (in-house tested).

RRID
AB_2687187 (BioLegend Cat. No. 697601)
AB_2687187 (BioLegend Cat. No. 697602)

Antigen Details

Structure
480 amino acids, and predicted Mw is 52.6kDa.
Distribution

Mitochondrion inner membrane

Function
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain.
Interaction
The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Protein Synthesis
Molecular Family
Mitochondrial Markers
Antigen References

1. Ellinger J, et al. 2016, Oncotarget 7: 86490.
2. Ma SL, et al. 2016, J. Psychiatr. Res. 76:143

Gene ID
7384 View all products for this Gene ID
UniProt
View information about UQCRC1 on UniProt.org
Go To Top Version: 0    Revision Date: 05-12-2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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