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Purified anti-ZNF451 Antibody

Pricing & Availability
Clone
W19095A (See other available formats)
Regulatory Status
RUO
Other Names
ZATT, Zinc finger protein 451, Coactivator for steroid receptors, COASTER, E3 SUMO-protein transferase ZNF451, E3 SUMO-protein ligase ZNF451, Zinc Finger Protein Associated With TDP2 And TOP2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W19095A_PURE_-ZNF451_WB_031924
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-ZNF451 (clone W19095A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W19095A_PURE_-ZNF451_WB_031924
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-ZNF451 (clone W19095A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W19095A_PURE_-ZNF451_-1_IHC-P-_031924
    IHC staining of Purified anti-ZNF451 (clone W19095A) on formalin-fixed paraffin-embedded human breast cancer tissue. Following antigen retrieval using Tris-EDTA pH 9.0, the tissue was incubated with 5 μg/mL of Purified anti-ZNF451 (clone W19095A) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
  • C. W19095A_PURE_-ZNF451_-2_IHC-P-_031924
    IHC staining of Purified anti-ZNF451 (clone W19095A) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA pH 9.0, the tissue was incubated with 5 µg/mL of Purified anti-ZNF451 (clone W19095A) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
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635151 25 µg 136€
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635152 100 µg 348€
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Description

ZNF451, or Zinc finger protein 451, exhibits E3 SUMO ligase activity along with zinc finger domains that regulate biological processes. These processes include sumoylation, TGF-β signaling, and the stability of promyelocytic leukemia (PML) bodies within the nucleus. ZNF451 contains sumo-related domains which enables SUMO-protein ligase activity and interaction with SUMO 2. In mouse models, the sumoylation of PML proteins by ZNF451 decreases the stability of PML bodies. In addition, in response to cellular stressors such as topoisomerase 2 (TOP2) activity and DNA damage, ZNF451 binds to TOP2 leading to the recruitment of TDP2. This recruitment facilitates the unloading of the TOP2 cleavage complex to regulate DNA repair mechanisms. The zinc finger domains of ZNF451 effectively bind to SMAD2/3/4 proteins and attenuate upstream TGF-β signals. This interaction results in the repression of transcriptional activity, thereby influencing downstream cellular processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human ZNF451
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W19095A) is not suitable for immunocytochemistry (ICC).

For immunohistochemistry (IHC), the recommended antigen retrieval buffer is either Tris-EDTA pH 9.0 (10 mM Tris, 1 mM EDTA) or Citrate Buffer, 10X (Cat. No. 420902).

RRID
AB_3106131 (BioLegend Cat. No. 635151)
AB_3106131 (BioLegend Cat. No. 635152)

Antigen Details

Structure
Forms a homooligomer
Distribution

Nuclear (promyelocytic leukemia body or nucleoplasm)

Function
Sumoylation, DNA repair
Interaction
Interacts with SUMO1, SUMO2, UBE2I/UBC9, SMAD2, SMAD3, SMAD4, ubiquitin, and SIMC1
Cell Type
Embryonic Stem Cells, Leukemia
Biology Area
DNA Repair/Replication, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Ligases
Antigen References
  1. Karvonen U, et al. 2008. J Mol Biol. 382:585-600. 
  2. Feng Y, et al. 2014. J Biol Chem. 289:2072-83.
  3. Cappadocia L, et al. 2015. Nat Struct Mol Biol. 22:968-75.
  4. Koidl S, et al. 2016. Int J Biochem Cell Biol. 79:478-487.
  5. Schellenberg MJ, et al. 2017. Science. 357:1412-1416.
Gene ID
26036 View all products for this Gene ID
UniProt
View information about ZNF451 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03-19-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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