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Recombinant Human BMP-5 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Bone morphogenetic protein 5
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595402 10 µg 175€
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Description

Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Nervous system development involves several BMPs including BMP-2, 4, 5, and 7. BMP-5 is widely expressed in the nervous system throughout development and into adulthood, and BMP-5 induces multiple dendritic processes to sympathetic neurons in culture. BMP-5 plays an important role in fracture healing during the stage of cartilage formation and periosteal response. In addition, BMP-4 and BMP-5 are expressed in normal synovial tissue and have been found to be decreased in osteoarthritis and rheumatoid arthritis; therefore, it has been speculated that these BMPs are important in joint homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human BMP-5, amino acids (Ala317-His454) (Accession# NM_021073), was expressed in CHO cells.
Molecular Mass
The 138 amino acid recombinant protein has a predicted molecular mass of approximately 15.6 kD. Recombinant human BMP-5 is a homodimeric disulfide-linked protein. The predicted N-terminal amino acid is Ala.
Purity
>95%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation
Lyophilized
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
BMP-5 induces alkaline phosphatase production on ATDC-5 cells. The expected ED50 = 0.5 - 1.0 µg/ml, corresponding to a specific activity of 1.0 - 2.0 x 103 unit/mg.
Application

Bioassay

Antigen Details

Structure
Growth factor, homodimer
Distribution

Chondrocytes, neurons.

Function
BMP-5 induces cartilaginous differentiation, chondrocyte cell proliferation, and cartilage matrix synthesis. It also regulates dendritic growth in sympathetic neurons. Noggin and follistatin inhibit the dendrite-promoting activity of BMP-5.
Interaction
Chondroblasts, osteoblasts, neurons.
Ligand/Receptor
Type I receptors BMPR-IA (ALK-3) and type II receptors.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Beck HN, et al. 2001. BMC Neurosci. 2:12.
2. Chen D, et al. 2004. Growth Factors 22:233.
3. Mailhot G, et al. 2008. J. Cell Physiol. 214:56.
4. Ai-Aql ZS, et al. 2008. J. Dent. Res. 87:107.
5. Gluhak-Heinrich J, et al. 2010. Bone 46:1533.

Gene ID
653 View all products for this Gene ID
UniProt
View information about BMP-5 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 2    Revision Date: 04-14-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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