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- Regulatory Status
- RUO
- Other Names
- Follistatin-like 1, Follistatin-like protein 1, follistatin-relatedprotein 1, Follistatin-related Protein (FRP), FRPFLJ52277, FSL1,FSTL1, TSC-36
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human BMP4 binds to immobilized recombinant human FSTL1 in a dose-dependent manner. EC50 of this binding is 6-48 ng/mL. -
Stability testing for recombinant human FSTL1. Recombinant human FSTL1 was aliquoted in PBS, pH 7.2, at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to human BMP4 in a dose-dependent manner. The EC50 for this effect is 6-48 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 588504 | 25 µg | 196€ | ||||
| 588506 | 100 µg | 436€ | ||||
Follistatin like 1 (FSTL1) or follistatin related protein (FRP), first identified as a TGF-β1 inducible gene, encodes a secreted extracellular glycoprotein that contains a follistatin-like domain in its amino acid sequence. FSTL1 is synthesized and secreted in various tissues such as cardiac and smooth muscle tissue. FSTL1 has been reported to play a role in modulating biological processes such as vascularization, immunomodulation and signaling pathway. FSTL1 enhances endothelial function and promotes revascularization in ischemic tissue by eNOS (endothelial nitric oxide synthase) activation mechanism. Physical exercise can increase FSTL1 gene expression in skeletal muscle. Studies found that FSTL1 may promote glioma growth and regulate lung development through the BMP4/Smad1/5/8 signaling pathway. These studies have established FSTL1’s role as a BMP4 signaling antagonist. FSTL1 has been identified as a novel autoantigen of synovium in rheumatoid arthritis (RA). FSTL1 was found essential for the immunosuppressive action of MSCs (mesenchymal stem cells) on inflammatory macrophages during liver fibrotic therapy.
Product DetailsProduct Details
- Source
- Human FSTL1, amino acids Glu21-Ile308 (Accession # Q12841), with a Cterminal His tag, was expressed in CHO cells.
- Molecular Mass
- The 301 amino acid recombinant protein has a predicted molecular mass of approximately 34.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 45-55 kD in SDS-PAGE. The predicted N-terminal amino acid is Glu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per μg of cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human BMP4 binds to immobilized recombinant human FSTL1 in a dose-dependent manner. EC50 of this binding is 6-48 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Cardiac and smooth muscle cells
- Function
- Angiogenesis, regulation of the immune response, cell proliferation and differentiation
- Interaction
- Endothelial cells, cardiac myocytes, skeletal muscle cells and lung epithelial cells
- Ligand/Receptor
- BMP4, CD14 and DIP2A
- Bioactivity
- Recombinant human BMP4 binds to immobilized recombinant human FSTL1 in a dose-dependent manner. EC50 of this binding is 6-48 ng/mL.
- Cell Type
- Endothelial cells, Epithelial cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Soluble Receptors, Tumor Suppressors
- Antigen References
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- Tanaka, M, et al. 1998. Int. Immunol. 10:1305-14.
- Ouchi N, et al.2008. J. Biol. Chem. 283:32802-11.
- Geng Y, et al. 2011. Proc Natl Acad Sci. USA. 108:7058-7063.
- Chaly Y, et al. 2014. Immunol Res. 59:266-72.
- Liu R, et al. 2015. Mol Brain. 8:53.
- Bae K, et al. 2016. Oncotarget. 7:8076-18076-84.
- Jin X, et al. 2017. Cell Physiol Biochem. 44:1616-1628.
- Chiou J, et al. 2017. Sci Rep. 7:9830.
- Zheng X, et al. 2022. Stem Cell Res Ther. 13:403.
- Gene ID
- 11167 View all products for this Gene ID
- UniProt
- View information about FSTL1 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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