Recombinant Mouse IGFBP-5 (carrier-free)

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Regulatory Status
RUO
Other Names
IBP5, IGFBP5
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Product Citations
publications
IGFBP-5_CF_Mouse_RECOM_BA_042315
Mouse IGFBP-5 inhibits the proliferation of MCF-7 cells induced by mouse IGF-II.
  • IGFBP-5_CF_Mouse_RECOM_BA_042315
    Mouse IGFBP-5 inhibits the proliferation of MCF-7 cells induced by mouse IGF-II.
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751202 10 µg 175€
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751204 25 µg 278€
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Description

Seven IGFBPs has been described to modulate the IGF activity. IGFBPs transport IGFs, in which they may inhibit mitogenesis, differentiation, survival, and other IGF-stimulated events. IGFBPs structurally are characterized by three domains: the amino-terminal, the carboxi-terminal, and an intermedia or variable L-domain. Members of the IGFBP family exhibit 67–70% structural homology. The greatest homology among the IGFBPs is in the N- and C-terminal regions. Some IGFBPs bind to the extracellular matrix (IGFBP-2, -3, -5, and -6). In fact, a heparin-binding domain (HBD) has been identified in the C-terminal region of these binding proteins. IGFBP-5 is the most conserved IGFBP in mammals. Additionally, human and mouse IGFBP-5 are 97% identical. The IGFBPs bind IGFs with high affinity (kD ∼0.1 nM), and this binding restricts the interaction of IGFs to their receptors. The release of the IGFs from IGFBP-5 is mediated by proteolysis; it has been described that IGFBP-5 is cleaved by ADAM-12, ADAM-9, C1s, and PAPPs (pregnancy-associated plasma proteases) A and A2. IGFBP-5 plays a key role in tissue repair and regeneration. IGFBP-5 is overexpressed in fibrotic lung tissues from patients with idiopathic pulmonary fibrosis. In vitro, IGFBP-5 induces the production of ECM components while in vivo, IGFBP-5 initiates the development of dermal and pulmonary fibrosis. In addition, IGFBP-5 induces lung fibroblast and mononuclear cell migration in an IGF-I–independent manner. Also, IGFBP-5 plays an important role in mammary development, involution, and breast cancer metastasis. IGFBP-5 has nuclear localization sequence that mediates β-importin-dependent translocation to the nucleus, where it interacts with the vitamin D receptor and modulates the vitamin D response in osteoblasts.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse IGFBP-5, amino acids (Val15 - Glu271) (Accession# NM_010518 ), was expressed in 293E cells. The amino-terminal possesses a 9 His-tag.
Molecular Mass
The 278 amino acid recombinant protein has a predicted molecular mass of approximately 31 kD. The protein migrates approximately at 40-45 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in < 30% ACN and 0.1% TFA.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.15 — 0.9 µg/ml as determined by inhibition of MCF-7 cell proliferation induced by mouse IGF-II (30 ng/ml).
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

IGFBP-5 is expressed in a big range of cell types and tissues.

Function
IGFBP-5 regulates the activity of both IGF-I and IGF-II by preventing their binding to the receptors. IGFBP-5 regulates osteogenesis, muscle differentiation, and kidney development, among other processes.
Interaction
Variety of cells.
Ligand/Receptor
IGF-I, IGF-II, and nuclear vitamin D receptor (VDR).
Bioactivity
IGFBP-5 inhibits the proliferation of MCF-7 cells induced by IGF-II.
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
Antigen References

1. Schuller AG, et al. 1994. Mol. Cell Endocrinol. 104:57.
2. Overgaard MT, et al. 2001. J. Biol. Chem. 276:21849.
3. Mohan S, et al. 2002. Biochemistry 41:15394.
4. Beattie J, et al. 2006. Biochem. J. 395:1.
5. Schedlich LJ, et al. 2007. Mol. Endocrinol. 21:2378.
6. Akkiprik M, et al. 2008. Breast Cancer Res. 10:212.
7. Yasuoka H, et al. 2009. Am. J. Respir. Cell Mol. Biol. 41:179.
8. Nili M, et al. 2012. J. Biol. Chem. 287:1510.

Gene ID
16011 View all products for this Gene ID
UniProt
View information about IGFBP-5 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 04-24-2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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