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Spark Blue™ 488 FLAER

Pricing & Availability
Regulatory Status
RUO
Other Names
AER, Pro-aerolysin, FLAER, aerolysin, GPI anchor
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Product Citations
publications
Human_SPKBL488_FLAER_1_120823
Human peripheral blood leukocytes were stained with Spark Blue™ 488 FLAER (left). Unstained cells are shown in the right plot.
  • Human_SPKBL488_FLAER_1_120823
    Human peripheral blood leukocytes were stained with Spark Blue™ 488 FLAER (left). Unstained cells are shown in the right plot.
  • Human_SPKBL488_FLAER_2_120823
    A GPI anchor-negative mutant of the human T cell leukemia cell line, Jurkat, was stained with Spark Blue™ 488 FLAER only (left) or mixed with wild type Jurkat cells (right).
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567903 25 tests 68€
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567904 100 tests 172€
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Description

The bacterial toxin aerolysin has the remarkable ability to bind selectively and with high affinity to the glycophosphatidylinositol (GPI) anchors of cell surface proteins, causing lysis of normal but not GPI-deficient cells. FLAER is a fluorescently-labeled, inactive variant of aerolysin that does not cause lysis of cells. The reagent binds selectively to cell surface GPI-anchored proteins, but will not bind to cells that are missing GPI-anchors, such as cells with deficiency in any of the phosphatidylinositol glycan complementation groups (PIG) genes.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
E. coli
Formulation
Phosphate-buffered Solution, pH 7.2, containing 0.09% sodium azide and BSA
Preparation
Recombinant proaerolysin was conjugated with Spark Blue™ 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The Spark Blue™ 488 FLAER solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this protein is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Nakamura, et al. 2012. PLOS ONE.
  2. Nguyen, et al. 2018. Am J Hum Genet. 103(4): 602-11.
  3. Chen Y, et al. 2022. Exp Hematol Oncol. 11:1.
  4. Brodsky RA, et al. 2000. Am J Clin Pathol. 114:459-66.
  5. Parker C, et al. 2005. Blood. 106(12): 3699–709.

Antigen Details

Structure
After binding to GPI-anchored proteins in target membranes and proteolytic removal of the C-terminal propeptide, the protein assembles into a heptameric pre-pore complex.
Distribution

Microbial toxin from Aeromonas hydrophila, host cell membrane

Function
Microbial toxin from Aeromonas hydrophila
Interaction
Plasma membrane, GPI anchor
Cell Type
Hematopoietic stem and progenitors
Biology Area
Immunology
Antigen References
  1. Nakamura, et al. 2012. PLOS ONE.
  2. Nguyen, et al. 2018. Am J Hum Genet. 103(4): 602-11.
  3. Chen Y, et al. 2022. Exp Hematol Oncol. 11:1.
  4. Brodsky RA, et al. 2000. Am J Clin Pathol. 114:459-66.
  5. Parker C, et al. 2005. Blood. 106(12): 3699–709.
Gene ID
NA
UniProt
View information about FLAER on UniProt.org
Go To Top Version: 1    Revision Date: 12-08-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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