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- Regulatory Status
- RUO
- Other Names
- AER, Pro-aerolysin, FLAER, aerolysin, GPI anchor
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood leukocytes were stained with Spark Blue™ 488 FLAER (left). Unstained cells are shown in the right plot. -
A GPI anchor-negative mutant of the human T cell leukemia cell line, Jurkat, was stained with Spark Blue™ 488 FLAER only (left) or mixed with wild type Jurkat cells (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 567903 | 25 tests | 68€ | ||||
| 567904 | 100 tests | 172€ | ||||
The bacterial toxin aerolysin has the remarkable ability to bind selectively and with high affinity to the glycophosphatidylinositol (GPI) anchors of cell surface proteins, causing lysis of normal but not GPI-deficient cells. FLAER is a fluorescently-labeled, inactive variant of aerolysin that does not cause lysis of cells. The reagent binds selectively to cell surface GPI-anchored proteins, but will not bind to cells that are missing GPI-anchors, such as cells with deficiency in any of the phosphatidylinositol glycan complementation groups (PIG) genes.
Product DetailsProduct Details
- Source
- E. coli
- Formulation
- Phosphate-buffered Solution, pH 7.2, containing 0.09% sodium azide and BSA
- Preparation
- Recombinant proaerolysin was conjugated with Spark Blue™ 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The Spark Blue™ 488 FLAER solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this protein is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
-
- Nakamura, et al. 2012. PLOS ONE.
- Nguyen, et al. 2018. Am J Hum Genet. 103(4): 602-11.
- Chen Y, et al. 2022. Exp Hematol Oncol. 11:1.
- Brodsky RA, et al. 2000. Am J Clin Pathol. 114:459-66.
- Parker C, et al. 2005. Blood. 106(12): 3699–709.
Antigen Details
- Structure
- After binding to GPI-anchored proteins in target membranes and proteolytic removal of the C-terminal propeptide, the protein assembles into a heptameric pre-pore complex.
- Distribution
-
Microbial toxin from Aeromonas hydrophila, host cell membrane
- Function
- Microbial toxin from Aeromonas hydrophila
- Interaction
- Plasma membrane, GPI anchor
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Immunology
- Antigen References
-
- Nakamura, et al. 2012. PLOS ONE.
- Nguyen, et al. 2018. Am J Hum Genet. 103(4): 602-11.
- Chen Y, et al. 2022. Exp Hematol Oncol. 11:1.
- Brodsky RA, et al. 2000. Am J Clin Pathol. 114:459-66.
- Parker C, et al. 2005. Blood. 106(12): 3699–709.
- Gene ID
- NA
- UniProt
- View information about FLAER on UniProt.org
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