Spark Blue™ 574 anti-human CD10 Antibody, CD10, HI10a

Pricing & Availability

Clone: HI10a (See other available formats)
Regulatory Status: RUO
Workshop: V CD10.7
Other Names: Common acute lymphoblastic leukemia antigen (CALLA), Enkephalinase, Neutral endopeptidase, Neprilysin
Isotype: Mouse IgG1, κ
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Product Citations: publications

HI10a_SparkBlue574_CD10_Antibody_2_030724 — Human peripheral blood granulocytes were stained with anti-human CD10 (clone HI10a) Spark Blue™ 574 (filled histogram) or with mouse IgG1, κ Spark Blue™ 574 isotype control (open histogram).

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312249

25 tests

123€

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312250

100 tests

292€

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Description

CD10 is a 100 kD neutral endopeptidase and a member of the metalloprotease family. It is a type II transmembrane protein also known as common acute lymphoblastic leukemia antigen (CALLA), enkephalinase, and neprilysin. CD10 is expressed on B cell precursors, T cell precursors, and neutrophils. CD10 is involved in B cell development and has been shown to bind opioid enkephalins, bradykinin, angiotensins I and II, and other biologically active peptides.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin monkey, Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3097621 (BioLegend Cat. No. 312249)
AB_3097621 (BioLegend Cat. No. 312250)

Antigen Details

Structure: Type II transmembrane metalloprotease family, 100 kD
Distribution: B cell precursors, T cell precursors, neutrophils
Function: Zinc-binding metalloproteinase, B cell development
Ligand/Receptor: Biologically active peptides including opioid enkephalins, bradykinin, angiotensions I & II
Cell Type: B cells, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Shipp M, et al. 1993. Blood 82:1052.
2. Lu B, et al. 1995. J. Exp. Med. 181:2271.
Gene ID: 4311 View all products for this Gene ID
UniProt: View information about CD10 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

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Other Formats

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Description	Clone	Applications
Purified anti-human CD10	HI10a	FC,CyTOF®,IHC-F
PE anti-human CD10	HI10a	FC
PE/Cyanine5 anti-human CD10	HI10a	FC
FITC anti-human CD10	HI10a	FC
APC anti-human CD10	HI10a	FC
APC/Cyanine7 anti-human CD10	HI10a	FC
PE/Cyanine7 anti-human CD10	HI10a	FC
PerCP/Cyanine5.5 anti-human CD10	HI10a	FC
Brilliant Violet 421™ anti-human CD10	HI10a	FC
Brilliant Violet 510™ anti-human CD10	HI10a	FC
Brilliant Violet 605™ anti-human CD10	HI10a	FC
Purified anti-human CD10 (Maxpar® Ready)	HI10a	FC,CyTOF®
Brilliant Violet 711™ anti-human CD10	HI10a	FC
PE/Dazzle™ 594 anti-human CD10	HI10a	FC
APC/Fire™ 750 anti-human CD10	HI10a	FC
APC anti-human CD10	HI10a	FC
PE anti-human CD10	HI10a	FC
FITC anti-human CD10	HI10a	FC
TotalSeq™-A0062 anti-human CD10	HI10a	PG
TotalSeq™-C0062 anti-human CD10	HI10a	PG
APC/Fire™ 750 anti-human CD10	HI10a	FC
TotalSeq™-B0062 anti-human CD10	HI10a	PG
PE/Cyanine7 anti-human CD10	HI10a	FC
PerCP/Cyanine5.5 anti-human CD10	HI10a	FC
Brilliant Violet 785™ anti-human CD10	HI10a	FC
TotalSeq™-D0062 anti-human CD10	HI10a	PG
GMP APC anti-human CD10	HI10a	FC
GMP FITC anti-human CD10	HI10a	FC
GMP PE/Cyanine7 anti-human CD10	HI10a	FC
GMP PE anti-human CD10	HI10a	FC
PE/Dazzle™ 594 anti-human CD10	HI10a	FC
GMP PerCP/Cyanine5.5 anti-human CD10	HI10a	FC
Brilliant Violet 650™ anti-human CD10	HI10a	FC
GMP PE/Dazzle™ 594 anti-human CD10	HI10a	FC
Brilliant Violet 750™ anti-human CD10	HI10a	FC
Spark Violet™ 423 anti-human CD10	HI10a	FC
Spark YG™ 593 anti-human CD10	HI10a	FC
Spark Blue™ 574 anti-human CD10	HI10a	FC
PE/Fire™ 640 anti-human CD10	HI10a	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03-07-2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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