Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) Antibody, CD138, 281-2

Pricing & Availability

Clone: 281-2 (See other available formats)
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ
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Product Citations: publications

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285151

50 µg

269€

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue³ and formalin-fixed paraffin embedded tissue⁴and immunofluorescent staining^2,3.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662491 (BioLegend Cat. No. 285151)

Antigen Details

Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: NA
UniProt: View information about CD138 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD138 (Syndecan-1)	281-2	FC,ICC,IHC-P,IHC-F
PE anti-mouse CD138 (Syndecan-1)	281-2	FC
APC anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1)	281-2	FC
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1)	281-2	FC
Biotin anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Cyanine7 anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)	281-2	FC
APC/Cyanine7 anti-mouse CD138 (Syndecan-1)	281-2	FC
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1)	281-2	FC
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
PE/Cyanine5 anti-mouse CD138 (Syndecan-1)	281-2	FC
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 700 anti-mouse CD138 (Syndecan-1)	281-2	FC
Spark Red™ 718 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™)	281-2	FC
Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™)	281-2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09-06-2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.