- Clone
- RB6-8C5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Gr-1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Raised against granulocytes of mouse origin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.
The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.
Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436). - Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
-
- Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
- Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
- Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
- Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
- Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
- Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
- Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
- Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
- Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
- Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
- Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
- Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
- Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
- Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
- Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
- Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
- Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
- Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
- Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
- Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
- Collins C, et al. 2014. PNAS. 111:9899. PubMed
- Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
- Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
- Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
- Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
- Distel E, et al. 2014. Circ Res. 115:759. PubMed
- Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
- Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
- Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
- Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
- Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
- RRID
-
AB_3662523 (BioLegend Cat. No. 285183)
Antigen Details
- Structure
- 21-25 kD
- Distribution
-
Granulocytes, monocytes
- Cell Type
- Granulocytes, Monocytes, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125. - Gene ID
- NA
- UniProt
- View information about Ly-6G Ly-6C on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All Ly-6G/Ly-6C Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse Ly-6G/Ly-6C (Gr-1)
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Biotin anti-mouse Ly-6G/Ly-6C (Gr-1)
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FITC anti-mouse Ly-6G/Ly-6C (Gr-1)
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PE anti-mouse Ly-6G/Ly-6C (Gr-1)
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PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
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PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)
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PerCP anti-mouse Ly-6G/Ly-6C (Gr-1)
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Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
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Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready)
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PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1)
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APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1)
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TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1)
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TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1)
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TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1)
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APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody
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Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1)
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Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Spark PLUS UV395™ anti-mouse Ly-6G/Ly-6C (Gr-1)
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Spark Red™ 718 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™)
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