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- TER-119 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-76
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Day-14 fetal liver cells from a C57BL/6 mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
-
- Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
- Vannucchi AM, et al. 2000. Blood 95:2559.
- Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
- Grisendi S, et al. 2005. Nature 437:147. (FC)
- Bourdeau A, et al. 2007. Blood 109:4220.
- Chappaz S, et al. 2007. Blood 110:3862. (FC)
- Heuser M, et al. 2007. Blood 110:1639. (FC)
- Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
- RRID
-
AB_3662526 (BioLegend Cat. No. 285186)
Antigen Details
- Structure
- Associated with glycophorin A, 52 kD
- Distribution
-
Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
- Cell Type
- Erythrocytes
- Biology Area
- Immunology
- Antigen References
-
1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5. - Gene ID
- NA
- UniProt
- View information about TER-119 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
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Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All TER-119 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse TER-119/Erythroid Cells
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Biotin anti-mouse TER-119/Erythroid Cells
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FITC anti-mouse TER-119/Erythroid Cells
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PE anti-mouse TER-119/Erythroid Cells
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PE/Cyanine5 anti-mouse TER-119/Erythroid Cells
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Purified anti-mouse TER-119/Erythroid Cells
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Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells
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Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells
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Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells
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PE/Cyanine7 anti-mouse TER-119/Erythroid Cells
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APC/Cyanine7 anti-mouse TER-119/Erythroid Cells
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PerCP anti-mouse TER-119/Erythroid Cells
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PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells
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Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells
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Pacific Blue™ anti-mouse TER-119/Erythroid Cells
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Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells
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Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells
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Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells
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Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)
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PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells
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Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells
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TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells
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APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells
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TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells
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TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells
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Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells
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Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells
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APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells
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Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody
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Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells
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Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™)
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