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Spark Red™ 718 anti-human CD14 Antibody

Pricing & Availability
Clone
S18004B (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
S18004B_Spark-RED-718_CD14_1_013024
Human peripheral blood monocytes were stained with anti-human CD14 (clone S18004B) Spark Red™ 718 (filled histogram). Open histogram represents unstained cells.
  • S18004B_Spark-RED-718_CD14_1_013024
    Human peripheral blood monocytes were stained with anti-human CD14 (clone S18004B) Spark Red™ 718 (filled histogram). Open histogram represents unstained cells.
  • S18004B_Spark-RED-718_CD14_2_013024
    Human peripheral blood monocytes were stained with anti-human HLA-DR Brilliant Violet 421™ and anti-human CD14 (clone S18004B) Spark Red™ 718 (left) or stained with anti-human HLA-DR Brilliant Violet 421™ only (right).
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399217 25 tests 82€
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399218 100 tests 196€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.

Excitation Laser
Red Laser (633 nm)
RRID
AB_3097149 (BioLegend Cat. No. 399217)
AB_3097149 (BioLegend Cat. No. 399218)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Interaction
Monocytes, Macrophages, Granulocytes, Neutrophils
Ligand/Receptor
LPS
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 1    Revision Date: 07-18-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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