Spark Violet™ 538 anti-human CD303 (BDCA-2) Antibody, CD303, 201A

Pricing & Availability

Clone: 201A (See other available formats)
Regulatory Status: RUO
Other Names: CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype: Mouse IgG2a, κ
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Product Citations: publications

201A_SparkViolet538_CD303_Antibody_053024 — Human peripheral mononuclear cells were stained with anti-human CD123 (clone 6H6) Brilliant Violet 650™ and anti-human CD303 (BDCA-2) (clone 201A) Spark Violet™ 538 (left) or mouse IgG2a, κ Spark Violet™ 538 isotype control (right). Data shown is gated on the lymphocyte and monocyte populations.

Compare all formats See Spark Violet™ 538 spectral data

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354249

25 tests

133€

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354250

100 tests

295€

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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3662261 (BioLegend Cat. No. 354249)
AB_3662261 (BioLegend Cat. No. 354250)

Antigen Details

Structure: Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution: Plasmacytoid dendritic cells
Function: Antigen capture, inhibition of IFN α/β production
Cell Type: Dendritic cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.
Gene ID: 170482 View all products for this Gene ID
UniProt: View information about CD303 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD303 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD303 (BDCA-2)	201A	FC
Purified anti-human CD303 (BDCA-2)	201A	FC
PE anti-human CD303 (BDCA-2)	201A	FC
FITC anti-human CD303 (BDCA-2)	201A	FC
PerCP/Cyanine5.5 anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 421™ anti-human CD303 (BDCA-2)	201A	FC
PE/Cyanine7 anti-human CD303 (BDCA-2)	201A	FC
Purified anti-human CD303 (BDCA-2) (Maxpar® Ready)	201A	FC,CyTOF®
Alexa Fluor® 647 anti-human CD303 (BDCA-2)	201A	FC
Biotin anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 785™ anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 605™ anti-human CD303 (BDCA-2)	201A	FC
PE/Dazzle™ 594 anti-human CD303 (BDCA-2)	201A	FC
Alexa Fluor® 700 anti-human CD303 (BDCA-2)	201A	FC
Alexa Fluor® 488 anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 510™ anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 711™ anti-human CD303 (BDCA-2)	201A	FC
APC/Fire™ 750 anti-human CD303 (BDCA-2)	201A	FC
APC/Cyanine7 anti-human CD303 (BDCA-2)	201A	FC
TotalSeq™-A0370 anti-human CD303 (BDCA-2)	201A	PG
TotalSeq™-C0370 anti-human CD303 (BDCA-2)	201A	PG
TotalSeq™-B0370 anti-human CD303 (BDCA-2)	201A	PG
TotalSeq™-D0370 anti-human CD303 (BDCA-2)	201A	PG
Spark Blue™ 550 anti-human CD303 (BDCA-2) (Flexi-Fluor™)	201A	FC
Spark Red™ 718 anti-human CD303 (BDCA-2) (Flexi-Fluor™)	201A	FC
APC/Fire™ 810 anti-human CD303 (BDCA-2)	201A	FC
Spark Violet™ 538 anti-human CD303 (BDCA-2)	201A	FC
PerCP/Fire™ 806 anti-human CD303 (BDCA-2)	201A	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05-30-2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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