Loading...

Spark YG™ 570 anti-β-Amyloid, 1-16 Antibody

Pricing & Availability
Clone
6E10 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
6E10_SparkYG570_B-Amyloid_1-16_Antibody_040320
IHC staining of Spark YG™ 570 anti-β-Amyloid, 1-16 antibody (clone 6E10) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using 88% formic acid, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with fluoromount G with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
  • 6E10_SparkYG570_B-Amyloid_1-16_Antibody_040320
    IHC staining of Spark YG™ 570 anti-β-Amyloid, 1-16 antibody (clone 6E10) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using 88% formic acid, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with fluoromount G with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
Compare all formats See Spark YG™ 570 spectral data
Cat # Size Price Quantity Check Availability Save
803025 25 µg 104€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
803026 100 µg 259€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13.

Application References
  1. Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed
  2. Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed
  3. Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed
  4. Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed
  5. Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed
  6. Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed
  7. Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed
  8. Sandoval-Hernandez A, et al. 2015. PLoS One. 10: 0145467. (IHC-F)
  9. Kumar R, et al. 2016. Brain. 139:174-92 (WB)
  10. Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB)
  11. Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed
  12. Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed
  13. Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed
  14. Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed
RRID
AB_2861077 (BioLegend Cat. No. 803025)
AB_2861077 (BioLegend Cat. No. 803026)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secr
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-16 on UniProt.org
Go To Top Version: 2    Revision Date: 03-12-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account