- Clone
- HI30 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N816
- Other Names
- LCA, T200
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- TGCAATTACCCGGAT
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
304089 | 10 µg | 296€ |
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding. - Additional Product Notes
-
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes. - Application References
-
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Kishihara K, et al. 1993. Cell 74:143.
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
- Nagano M, et al. 2007. Blood 110:151.
- Jiang Q, et al. 2008. Blood 112:2858. PubMed
- Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
- Lee J, et al. 2015. J Exp Med. 212:385. PubMed
- Breton G, et al. 2015. J Exp Med. 212:401. PubMed
- Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- RRID
-
AB_2941470 (BioLegend Cat. No. 304089)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, not expressed in circulating erythrocytes or platelets
- Function
- TCR and BCR mediated activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas M. 1989. Annu. Rev. Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
Other Formats
View All CD45 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45
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Biotin anti-human CD45
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FITC anti-human CD45
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PE anti-human CD45
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PE/Cyanine5 anti-human CD45
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Purified anti-human CD45
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APC/Cyanine7 anti-human CD45
-
PE/Cyanine7 anti-human CD45
-
Alexa Fluor® 488 anti-human CD45
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Alexa Fluor® 647 anti-human CD45
-
Pacific Blue™ anti-human CD45
-
Alexa Fluor® 700 anti-human CD45
-
PerCP anti-human CD45
-
PerCP/Cyanine5.5 anti-human CD45
-
Brilliant Violet 421™ anti-human CD45
-
Brilliant Violet 570™ anti-human CD45
-
Brilliant Violet 510™ anti-human CD45
-
Brilliant Violet 605™ anti-human CD45
-
Brilliant Violet 650™ anti-human CD45
-
Purified anti-human CD45 (Maxpar® Ready)
-
Brilliant Violet 785™ anti-human CD45
-
Brilliant Violet 711™ anti-human CD45
-
PE/Dazzle™ 594 anti-human CD45
-
Alexa Fluor® 594 anti-human CD45
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APC/Fire™ 750 anti-human CD45
-
Pacific Blue™ anti-human CD45
-
APC anti-human CD45
-
PE/Cyanine7 anti-human CD45
-
PE/Dazzle™ 594 anti-human CD45
-
TotalSeq™-A0391 anti-human CD45
-
TotalSeq™-B0391 anti-human CD45
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TotalSeq™-C0391 anti-human CD45
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PerCP/Cyanine5.5 anti-human CD45
-
APC/Fire™ 750 anti-human CD45
-
PE/Fire™ 640 anti-human CD45
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APC/Fire™ 810 anti-human CD45
-
Spark YG™ 570 anti-human CD45
-
PE/Fire™ 700 anti-human CD45
-
FITC anti-human CD45
-
PerCP anti-human CD45
-
Alexa Fluor® 660 anti-human CD45 Antibody
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Spark Violet™ 538 anti-human CD45
-
Spark YG™ 593 anti-human CD45
-
GMP APC/Fire™ 750 anti-human CD45
-
Spark Violet™ 538 anti-human CD45
-
GMP APC anti-human CD45
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Spark UV™ 387 anti-human CD45
-
GMP Pacific Blue™ anti-human CD45
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GMP PerCP anti-human CD45
-
GMP FITC anti-human CD45
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PE anti-human CD45
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GMP PE/Dazzle™ 594 anti-human CD45
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GMP PerCP/Cyanine5.5 anti-human CD45
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Spark Blue™ 515 anti-human CD45
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TotalSeq™-D0391 anti-human CD45
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GMP PE/Cyanine7 anti-human CD45
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Spark Violet™ 500 anti-human CD45
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GMP PE anti-human CD45
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PE/Fire™ 810 anti-human CD45
-
Spark PLUS UV395™ anti-human CD45
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Spark NIR™ 685 anti-human CD45
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Spark Violet™ 423 anti-human CD45
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