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Ultra-LEAF™ Purified anti-human CD40 Antibody

Pricing & Availability
Clone
W17212H (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor receptor superfamily member 5, TNFRSF5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17212H_ULEAF_CD40_Antibody_1_083019_updated
Recombinant biotinylated hCD40L (Black circles) binds to immobilized recombinant hCD40 (Cat. No. 777206). Anti-hCD40 antibody (clone W17212H, Purple squares) inhibits the binding in a dose-dependent manner whereas the Isotype control (Cat. No. 400666, Green triangles) doesn’t have the effect. This antibody blocks the binding of 80 ng/mL hCD40L biotinylated protein to 1 µg/mL immobilized recombinant hCD40 with an ND50 = 0.2 - 1 µg/mL.
  • W17212H_ULEAF_CD40_Antibody_1_083019_updated
    Recombinant biotinylated hCD40L (Black circles) binds to immobilized recombinant hCD40 (Cat. No. 777206). Anti-hCD40 antibody (clone W17212H, Purple squares) inhibits the binding in a dose-dependent manner whereas the Isotype control (Cat. No. 400666, Green triangles) doesn’t have the effect. This antibody blocks the binding of 80 ng/mL hCD40L biotinylated protein to 1 µg/mL immobilized recombinant hCD40 with an ND50 = 0.2 - 1 µg/mL.
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668103 100 µg 216€
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668104 1 mg 572€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD40, also known as TNFRSF5, is a type I transmembrane protein that belongs to the TNF receptor family (TNFR). CD40 is expressed on B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34+ hematopoietic progenitors. The interaction between CD40 and its ligand TNFSF5/CD40L leads to the clustering of CD40 and the recruitment of TNFR associated factors (TRAFs), including TRAF1, 2, 3, 5 and 6. TRAF1, 2, 5 and 6 serve as positive regulators while TRAF3 is a negative regulator. CD40/CD40L interaction triggers downstream signaling on both cells, including upregulation of CD40 and cell survival on dendritic cells, upregulation of co-stimulatory molecules, CD80 and CD86 on T cells, and production of inflammatory cytokines. In addition, CD40 regulates B cell development/maturation, Ig isotype switching and in combination with other signals such as IL-4, promotes proliferation. It has been reported that a transcriptional factor, AKNA, can coordinately regulate the expression of CD40 and CD40L. Dysregulation of CD40 is associated with several diseases, such as hyper-IgM immunodeficiency type 3 (HIGM3) and Hodgkin's disease. Targeting of CD40/CD40L for cancer therapy is being evaluated in clinical trials, including anti-CD40 agonist antibodies and soluble recombinant CD40L. Several isoforms have been identified in humans and mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD40 Recombinant Protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between hCD40 and hCD40L. The ND50 range: 0.2 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Korniotis S, et al. 2022. J Cell Sci. 135:. PubMed
RRID
AB_2814510 (BioLegend Cat. No. 668103)
AB_2814510 (BioLegend Cat. No. 668104)

Antigen Details

Structure
Disulfide bond-linked homodimer
Distribution

B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34+ hematopoietic progenitors, primary carcinomas.

Function
CD40 regulates B cell development/maturation, Ig isotype switching proliferation. CD40 is also involved in T cell priming and inflammatory cytokine production.
Interaction
TRAF1, 2, 3, 5, 6
Ligand/Receptor
TNFSF5/CD40L
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Platelets
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Ridge JP, et al. 1998. Nature 393:474.
  2. Schoenberger SP, et al. 1998. Nature 393:480.
  3. Bremer E. 2013. ISRN Oncol. 2013:371854.
  4. Siddiqa A, et al. 2001. Nature. 410: 383.
  5. Durandy A, et al. 2005. Immunol Rev. 203:67.
  6. O'Grady JT, et al. 1994. Am J Pathol. 144:21.
  7. Karaca NE, et al. 2012. Scand J Immunol. 76:21.
  8. Tone M, et al. 2001. Proc Natl Acad Sci USA. 98:1751.
  9. Eshel D, et al. 2008. Mol Immunol. 46:250.
  10. Hostager BS, et al. 2000. J Biol Chem. 275:15392.
  11. Elgueta R, et al. 2009. Immunol Rev. 229:152.
Gene ID
958 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08-23-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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