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Ultra-LEAF™ Purified anti-human Ephrin-A1 Antibody

Pricing & Availability
Clone
A16015A (See other available formats)
Regulatory Status
RUO
Other Names
EFNA1, EPLG1, TNFAIP4, LERK1, EFL1, B61
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16015A_ULEAF_Ephrin-A1_Antibody_1_020524
Biotinylated recombinant human Ephrin-A1-Fc Chimera (black circles) binds to immobilized recombinant human EphA1-Fc (Cat. No. 791606). Ultra-LEAF™ purified anti-human Ephrin-A1 (Clone A16015A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have effect. This antibody blocks the binding of 0.1 µg/mL biotinylated recombinant human Ephrin-A1-Fc chimera to 5 µg/mL immobilized recombinant human EphA1-Fc. ND50 range: 0.02 - 0.14 µg/mL.
  • A16015A_ULEAF_Ephrin-A1_Antibody_1_020524
    Biotinylated recombinant human Ephrin-A1-Fc Chimera (black circles) binds to immobilized recombinant human EphA1-Fc (Cat. No. 791606). Ultra-LEAF™ purified anti-human Ephrin-A1 (Clone A16015A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have effect. This antibody blocks the binding of 0.1 µg/mL biotinylated recombinant human Ephrin-A1-Fc chimera to 5 µg/mL immobilized recombinant human EphA1-Fc. ND50 range: 0.02 - 0.14 µg/mL.
Cat # Size Price Quantity Check Availability Save
629753 100 µg 288€
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629754 1 mg 964€
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Description

Ephrins are cell surface molecules with a wide range of biological functions that are involved in both embryogenesis and adult life. This includes important roles in boundary formation, cell migration, repulsive axon guidance, and the regulation of neuronal growth cone development. The ephrin family consists of eight members. Ephrin-A1 through A5 are anchored to the cell membrane via GPI linkage, while ephrin-B1 through B3 are transmembrane proteins. The interaction between ephrin ligands and its cognate Eph receptors involve both forward and reverse signaling. Ephrin-A1 was originally isolated in conditioned media from HUVEC cells treated with TNF-α, and the gene was found to be induced by TNF-α in these cells. Ephrin-A1 is broadly expressed on endothelial cells and epithelial cells, but it can also be released as a soluble molecule. Ephrin-A1 and its primary receptor EphA2 play a vital role in normal angiogenesis and tumor neovascularization. Ephrin-A1 expressed on tumor cells inhibits tumor cell proliferation and migration, but also supports tumor growth by promoting angiogenesis. Ephrin-A1 is also expressed on resting CD4+ T cells and its expression is down regulated in asthma patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Ephrin-A1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.1 µg/mL biotinylated human Ephrin-A1-Fc chimera to 5 µg/mL immobilized recombinant human EphA1-Fc chimera. ND50 range: 0.02 - 0.14 µg/mL.

Antigen Details

Structure
Monomer and Homodimer
Distribution

Widely expressed in differentiated epithelial cells in bone marrow, spleen, thymus, and testes. Also expressed on CD4+ T cells and a subpopulation of CD19+ B cells

Function
Regulation of angiogenesis, axongenesis, endothelial cell migration, integrin mediated adhesion, notochord formation, and negative regulation of epithelial to mesenchymal transition
Interaction
Endothelial cells, epithelial cells, resting CD4+ T cells
Ligand/Receptor
EphA2
Cell Type
Endothelial cells, Macrophages, NK cells, T cells
Biology Area
Angiogenesis, Cell Biology, Immuno-Oncology, Neurodegeneration, Neuroscience
Antigen References
  1. Lisabeth EM, et al. 2013. Cold Spring Harb Perspect Biol. USA. 5:9.
  2. Pasquale EB. 2008, Cell. 133:38-52.
  3. Hirai H, et al. 1987. Science. 238:1717-20.
  4. Aasheim HC, et al. 2005. Blood. 105:2869-76.
  5. Coulthard MG, et al. 2001. Growth Factors. 18:303-17.
  6. Ieguchi K and Maru Y. 2019. Cancer Sci. 3:841.
  7. Inokuchi M, et al. 2018. Anticancer Res. 3:1685
  8. Jin Y, et al. 2018. Mol Med. Rep. 17:5409.
  9. Chu M and Zhang C. 2018. Sci Rep. 1:1539
  10. Darling TK and Lamb TJ. 2019. Front Immunol. 10:01473.
Gene ID
1942 View all products for this Gene ID
UniProt
View information about Ephrin-A1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02-05-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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