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Ultra-LEAF™ Purified anti-mouse CXCL9 (MIG) Antibody

Pricing & Availability
Clone
A16072G (See other available formats)
Regulatory Status
RUO
Other Names
MIG, Monokine induced by interferon gamma, SCYB9, Small inducible cytokine, subfamily B, member 9
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
A16072G_ULEAF_CXCL9_Antibody_060121
Recombinant mouse CXCL9 (MIG) (Cat. No. 578204) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL9 (MIG) antibody (clone A16072G) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL9 (MIG) on mouse Baf3-mouse CXCR3 cells in a dose-dependent manner whereas the rat IgG2a, λ isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.15 - 1 µg/mL.
  • A16072G_ULEAF_CXCL9_Antibody_060121
    Recombinant mouse CXCL9 (MIG) (Cat. No. 578204) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL9 (MIG) antibody (clone A16072G) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL9 (MIG) on mouse Baf3-mouse CXCR3 cells in a dose-dependent manner whereas the rat IgG2a, λ isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.15 - 1 µg/mL.
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942603 100 µg 260€
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942604 1 mg 836€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CXCL9 is an inflammatory chemokine initially identified by differential screening of a cDNA library from limphokine-activated macrophages. CXCL9 is a CXC chemokine and member of the non-ELR (lacking a Glu-Leu-Arg motif in the N-terminal region) CXC chemokine family. CXCL9 shares the CXCR3 receptor with CXCL10 and CXCL11, and these ligands differ in their receptor-binding and activating properties; CXCL11 is more potent than CXCL10 and CXCL10 is more potent than CXCL9. CXCL9 plays a key role in leukocyte trafficking, acting on activated CD4+ Th1 cells, CD8+ T cells, IL-2 activated T lymphocytes, and NK cells. In addition, CXCL9 enhances T lymphocyte function in alloimmune response; CXCL9 induces T cells proliferation and cytokine production in an experimental model of cardiac allograft vasculopathy. Also, CXCL9 has angiostatic activity. Additionally, it has been hypothesized that CXCL9, CXCL10, and CXCL11 contribute to host defense against inhalational anthrax in a murine model of infection. CXCL9 is induced by cytokines, particularly IFN-γ during infection, injury, or immunoinflammatory responses, and it can be inactivated by CD26/dipeptidil peptidase IV after truncation by this protease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CXCL9
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL9 (MIG) (Cat. No. 578204) on mouse Baf3-mCXCR3 transfectant chemoattractant cells. ND50 range: 0.15 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892505 (BioLegend Cat. No. 942603)
AB_2892505 (BioLegend Cat. No. 942604)

Antigen Details

Structure
Chemokine
Distribution

CXCL9 is secreted by monocytes, macrophages, APC, eosinophils, endothelial cells, and B cells. Also, CXCL9 is secreted by fibroblast in inflammatory conditions.

Function
Induce chemotaxis, promote differentiation and multiplication of leukocytes, cause tissue extravasation, enhance CNS immunity; CXCL9/CXCR3 receptor regulates immune cell migration, differentiation, and activation
Interaction
Activated T lymphocytes, CD4 Th1, CD8+, NK, and NKT cells
Ligand/Receptor
CXCR3
Antigen References

1. Loetscher M, et al. 1996. J. Exp. Med. 184:963. 
2. Lambeir AM, et al. 2001. J. Biol. Chem. 276:29839. 
3. Whiting D, et al. 2004. J. Immunol. 172:7417. 
4. Gorbachev AV, et al. 2007. J. Immunol. 178:2278. 
5. Rosenblum JM, et al. 2010. J. Immunol. 184:3450. 
6. Crawford MA, et al. 2010. PLoS Pathog 6:e1001199.

Gene ID
17329 View all products for this Gene ID
UniProt
View information about CXCL9 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09-26-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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