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Alexa Fluor® 647 anti-STAT3 Phospho (Tyr705) Antibody

Pricing & Availability
Clone
13A3-1 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype
Mouse IgG1, κ
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Product Citations
publications
13A3-1_AF647_STAT3_Phospho_Tyr705_Antibody_1_032416
Human whole blood was stimulated with (top), or without (bottom) IL-6 for 15 minutes, and then treated with RBC Lysis/Fixation Solution (10X), permeabilized with True-Phos™ Perm Buffer, then stained with CD3 PE and STAT3 Phospho (Tyr705) (clone 13A3-1) Alexa Fluor® 647.
  • 13A3-1_AF647_STAT3_Phospho_Tyr705_Antibody_1_032416
    Human whole blood was stimulated with (top), or without (bottom) IL-6 for 15 minutes, and then treated with RBC Lysis/Fixation Solution (10X), permeabilized with True-Phos™ Perm Buffer, then stained with CD3 PE and STAT3 Phospho (Tyr705) (clone 13A3-1) Alexa Fluor® 647.
  • 13A3-1_AF647_STAT3_Phospho_Tyr705_Antibody_2_032416
Compare all formats See Alexa Fluor® 647 spectral data See supplemental data...
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651007 25 tests 137€
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651008 100 tests 306€
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Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated modified synthetic peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
 

Product Citations
  1. Chen HY, et al. 2022. Elife. 11:. PubMed
  2. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  3. Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
  4. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2572085 (BioLegend Cat. No. 651007)
AB_2572085 (BioLegend Cat. No. 651008)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous.

Function
STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
Cell Sources
Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Regulation
The small GTPase Rac1 binds and regulates activity of STAT3.
Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho Tyr705 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07-27-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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