Loading...

APC anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
2B8_APC_1_072611
  • 2B8_APC_1_072611
  • 2B8_APC_2_072611
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
105811 25 µg 68€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
105812 100 µg 168€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Barkaway A, et al. 2021. Immunity. . PubMed
  2. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Lengefeld J, et al. 2021. Sci Adv. 7:eabk0271. PubMed
  5. Julien E, et al. 2021. Stem Cells. 39:636. PubMed
  6. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  7. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  8. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  9. Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed
  10. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  11. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  12. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  13. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  14. Guan H, et al. 2023. Int J Mol Sci. 24:. PubMed
  15. Elbasiony E, et al. 2023. NPJ Aging. 9:2. PubMed
  16. Ma S, et al. 2023. Front Cell Dev Biol. 11:1140554. PubMed
  17. Wang X, et al. 2023. Nature. 618:808. PubMed
  18. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  19. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  20. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  21. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  22. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  23. Chen CC, et al. 2020. Cancer Cell. 37:71. PubMed
  24. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  25. Zhu H, et al. 2018. Elife. 7:. PubMed
  26. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  27. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  28. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  29. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  30. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  31. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  32. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  33. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  34. Gao B, et al. 2018. Aging Cell. 3. PubMed
  35. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  36. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  37. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  38. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  39. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  40. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  41. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  42. Nguyen DH, et al. 2020. Nat Cell Biol. 22:1423. PubMed
  43. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  44. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  45. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  46. Ritter M, et al. 2020. Ann Hematol. 99:2329. PubMed
  47. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  48. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  49. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  50. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  51. Plein A, et al. 2018. Nature. 562:223. PubMed
  52. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  53. Widner DB, et al. 2021. J Cachexia Sarcopenia Muscle. 12:1079. PubMed
  54. Eisele AS, et al. 2022. Elife. 11:. PubMed
  55. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  56. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  57. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  58. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  59. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  60. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  61. Dong S, et al. 2021. Nature. 591:117. PubMed
  62. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  63. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  64. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  65. Umemoto T, et al. 2008. Biochem Biophys Res Commun. 365:176. PubMed
  66. Convente MR, et al. 2018. J Bone Miner Res. 33:269. PubMed
  67. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  68. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  69. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  70. Wang X, et al. 2020. Cell Res. 30:1109. PubMed
  71. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  72. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  73. Sibon D et al. 2019. Cell reports. 26(12):3246-3256 . PubMed
  74. Tsuchiya K, et al. 2019. Nat Commun. 10:2091. PubMed
  75. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  76. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  77. Gandhapudi S, et al. 2015. J Immunol. 194:3820. PubMed
  78. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  79. Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
  80. N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed
  81. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  82. Li W, et al. 2021. PLoS Pathog. 17:e1009462. PubMed
  83. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  84. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  85. Engblom C, et al. 2017. Science. 358:6367. PubMed
  86. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  87. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  88. Suzuki S, et al. 2021. Cell Reports. 34(7):108752. PubMed
  89. Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed
  90. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  91. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  92. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  93. Artinger E, et al. 2013. Proc Natl Acad Sci U S A. 110:12000. PubMed
  94. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  95. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  96. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  97. Koide S, et al. 2022. iScience. 25:103603. PubMed
  98. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  99. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  100. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  101. Brian BF 4th et al. 2019. Elife. 8 pii: e46043. PubMed
  102. Dhayade S, et al. 2020. Nutrients. 12:. PubMed
  103. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  104. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  105. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  106. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  107. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  108. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  109. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  110. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  111. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  112. Liu J, et al. 2012. Proc Natl Acad Sci U S A. 109:10018. PubMed
  113. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  114. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  115. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  116. Cui Y, et al. 2020. Eur J Pharmacol. 881:173233. PubMed
  117. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_313220 (BioLegend Cat. No. 105811)
AB_313220 (BioLegend Cat. No. 105812)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 2    Revision Date: 06-28-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account