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ELISA MAX™ Standard Set Mouse IL-6

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Regulatory Status
RUO
Other Names
IL-6 ELISA Kit
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Product Citations
publications
mouse il-6_122109
  • mouse il-6_122109
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431301 5 plates 212€
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IL-6 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-6 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-6 Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
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  4. Cao YW, et al. 2015. J Ethnopharmacol. 161:92. PubMed
Product Citations
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  14. Zhang Y, et al. 2022. Nat Commun. 13:2460. PubMed
  15. Nulty C, et al. 2021. Methods Mol Biol. 2283:175. PubMed
  16. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  17. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  18. Kuroishi T, et al. 2013. Toxicol Sci. 135:119. PubMed
  19. Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
  20. Aryal P, et al. 2021. J Lipid Res. 62:100108. PubMed
  21. Lopez-Millan B, et al. 2017. Exp Mol Med. 49:e290. PubMed
  22. Hwang J, et al. 2015. Sci Rep. 5: 15971. PubMed
  23. Zhou P, et al. 2018. Nat Commun. 9:1243. PubMed
  24. Zhao M, et al. 2018. Cell Death Dis. 9:582. PubMed
  25. Felgner S, et al. 2020. Elife. 9:00. PubMed
  26. Hsieh PS, et al. 2021. Exp Ther Med. 188:21. PubMed
  27. Duan S, et al. 2021. J Immunol. 206:2290. PubMed
  28. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  29. Zhou B, et al. 2019. MBio. 10. PubMed
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  33. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
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  35. Kolar SL, et al. 2019. JCI Insight. 4:5. PubMed
  36. Phillippe M, Adeli S, et al. 2017. PLoS One. 12(6):e0178845. PubMed
  37. Li HY, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:289. PubMed
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  56. Cao Y, et al. 2015. J Ethnopharmacol. 161:92. PubMed
RRID
AB_2883997 (BioLegend Cat. No. 431301)
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org
Go To Top Version: 2    Revision Date: 09-11-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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